OntologiesmiclassesMI:0096   
pull down
http://purl.obolibrary.org/obo/MI_0096

A specific affinity chromatography method where a molecule of interest (bait) is bound to a column, often via an affinity tag expressed as a protein fusion (GST, HIS tag and others) or chemically linked to the bait molecule . The molecule may be expressed or synthesised and purified first, often in an heterologous system, bound to the matrix at high concentration and then challenged with a solution or cellular extract containing the candidate partner molecules. Alternatively, a multi-molecular complex may be adsorbed to the resin and the retained binding molecules subsequently identified.

Related Synonyms
affinity capture
pulldown
class Information
has_obo_namespace

PSI-MI

in_subset

PSI-MI_slim

class Relations