Function and Biology Details
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this NMR entry.
Refinement method:
Hybrid distance geometry-dynamical simulated annealing and refinement protocol for monomer structure determination,
with 457 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 sequential, |i-j|=1; 95 medium range, 1<|i-j|<5;
41 long range, |i-j|>4), 19 distance restraints representing hydrogen
bonds (entered as 2 distances each), 25 phi- and 5 chi1-torsion angle restraints.
Molecular dynamical simulated annealing protocol for dimer structure determination, using
505 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 sequential, |i-j|=1;
95 medium range, 1<|i-j|<5; 25 long range, |i-j|>4; 38 inter-molecular; 26 ambiguous),
19 distance restraints representing hydrogen bonds (entered as 2 distances each),
25 phi- and 5 chi1-torsion angle restraints.
Expression system: Escherichia coli