› 1nc3

X-ray diffraction
2.2Å resolution

Crystal structure of E. coli MTA/AdoHcy nucleosidase complexed with formycin A (FMA)

Released:
DOI: 10.2210/pdb1nc3/pdb
PDB entry 1nc3 coloured by chain, front view.
PDB entry 1nc3 coloured by chain, side view.
PDB entry 1nc3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase inhibitor complexes provide insight into the conformational changes required for substrate binding and catalysis.
Lee JE, Cornell KA, Riscoe MK, Howell PL
J Biol Chem 278 8761-70 (2003)
PMID: 12496243

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AF12 (Residues: 1-232; Coverage: 100%)
Gene names: JW0155, b0159, mtn, mtnN, pfs, yadA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand FMC 2 x FMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 51.1Å b: 69.7Å c: 127.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.247
Expression system: Escherichia coli BL21

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Citations

2 review citations

Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.
Parveen et al. (2011)
1 more

6 mentions without citation

Human antibodies that neutralize HIV-1: identification, structures, and B cell ontogenies.
Kwong et al. (2012)
5 more