2is4

X-ray diffraction
2.6Å resolution

Crystal structure of UvrD-DNA-ADPNP ternary complex

Released:
Model geometry
Fit model/data
Source organism: Escherichia coli
Primary publication:
UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.
Cell 127 1349-60 (2006)
PMID: 17190599

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-115408 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA helicase II Chains: A, B
Molecule details ›
Chains: A, B
Length: 680 amino acids
Theoretical weight: 77.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03018 (Residues: 1-680; Coverage: 94%)
Gene names: JW3786, b3813, mutU, pdeB, rad, recL, uvrD
Sequence domains:
Structure domains:
25-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 104.112Å b: 96.779Å c: 111.044Å
α: 90° β: 93.85° γ: 90°
R-values:
R R work R free
0.215 0.215 0.256
Expression systems:
  • Escherichia coli
  • Not provided