2jba

X-ray diffraction
1.45Å resolution

PhoB response regulator receiver domain constitutively-active double mutant D53A and Y102C.

Released:
Model geometry
Fit model/data

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-142731 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphate regulon transcriptional regulatory protein PhoB Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFJ5 (Residues: 1-127; Coverage: 56%)
Gene names: JW0389, b0399, phoB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Phosphate regulon transcriptional regulatory protein PhoB Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 14.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFJ5 (Residues: 1-127; Coverage: 56%)
Gene names: JW0389, b0399, phoB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 44.907Å b: 47.631Å c: 59.703Å
α: 90° β: 99.34° γ: 90°
R-values:
R R work R free
0.189 0.189 0.204
Expression system: Escherichia coli BL21(DE3)