› 2ri7

X-ray diffraction
1.45Å resolution

Crystal structure of PHD finger-linker-bromodomain Y17E mutant from human BPTF in the H3(1-9)K4ME2 bound state

Released:
DOI: 10.2210/pdb2ri7/pdb
PDB entry 2ri7 coloured by chain, front view.
PDB entry 2ri7 coloured by chain, side view.
PDB entry 2ri7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger.
Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ
Mol Cell 28 677-91 (2007)
PMID: 18042461

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159403 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleosome-remodeling factor subunit BPTF Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12830 (Residues: 2865-3033; Coverage: 6%)
Gene names: BPTF, FAC1, FALZ
Sequence domains:
Structure domains:
Histone H3.1 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-10; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
Ligand IPA 1 x IPA
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 38.249Å b: 64.381Å c: 85.558Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.192
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

54 review citations

Histones: annotating chromatin.
Campos et al. (2009)
53 more

5 mentions without citation

Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger.
Li et al. (2007)
4 more