2ht4

X-ray diffraction
3.2Å resolution

Structure of the Escherichia coli ClC chloride channel Y445W mutant and Fab complex

Released:
Model geometry
Fit model/data

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
Ig-like domain-containing protein Chains: C, E
Molecule details ›
Chains: C, E
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
Fab fragment, Light chain Chains: D, F

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 234.007Å b: 95.94Å c: 175.327Å
α: 90° β: 133.2° γ: 90°
R-values:
R R work R free
0.24 0.239 0.261
Expression system: Escherichia coli BL21(DE3)