› 2zqy

X-ray diffraction
2.6Å resolution

T-state structure of allosteric L-lactate dehydrogenase from Lactobacillus casei

Released:
DOI: 10.2210/pdb2zqy/pdb
Model geometry
Fit model/data
PDB entry 2zqy coloured by chain, front view.
PDB entry 2zqy coloured by chain, side view.
PDB entry 2zqy coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Active and inactive state structures of unliganded Lactobacillus casei allosteric L-lactate dehydrogenase.
Arai K, Ishimitsu T, Fushinobu S, Uchikoba H, Matsuzawa H, Taguchi H
Proteins 78 681-94 (2010)
PMID: 19787773

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 326 amino acids
Theoretical weight: 35.57 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: P00343 (Residues: 1-326; Coverage: 100%)
Gene name: ldh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NO3 8 x NO3
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 64.337Å b: 143.984Å c: 144.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.265
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Sampling the conformational energy landscape of a hyperthermophilic protein by engineering key substitutions.
Colletier et al. (2012)
10 more

1 mention without citation

The core of allosteric motion in Thermus caldophilus L-lactate dehydrogenase.
Ikehara et al. (2014)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO