› 3fr7

X-ray diffraction
1.55Å resolution

ketol-acid reductoisomerase (KARI) in complex with Mg2+

Released:
DOI: 10.2210/pdb3fr7/pdb
PDB entry 3fr7 coloured by chain, front view.
PDB entry 3fr7 coloured by chain, side view.
PDB entry 3fr7 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Conformational changes in a plant ketol-acid reductoisomerase upon Mg(2+) and NADPH binding as revealed by two crystal structures.
Leung EW, Guddat LW
J Mol Biol 389 167-82 (2009)
PMID: 19362563

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketol-acid reductoisomerase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 525 amino acids
Theoretical weight: 57.38 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65XK0 (Residues: 54-578; Coverage: 91%)
Gene names: LOC_Os05g49800, OJ1735_C10.18, Os05g0573700
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 126.032Å b: 92.901Å c: 91.187Å
α: 90° β: 100.58° γ: 90°
R-values:
R R work R free
0.203 0.202 0.23
Expression system: Escherichia coli

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Citations

6 review citations

Interdependence of threonine, methionine and isoleucine metabolism in plants: accumulation and transcriptional regulation under abiotic stress.
Joshi et al. (2010)
5 more