› 3j81

Electron Microscopy
4Å resolution

CryoEM structure of a partial yeast 48S preinitiation complex

Released:
DOI: 10.2210/pdb3j81/pdb
PDB entry 3j81 coloured by chain, front view.
PDB entry 3j81 coloured by chain, side view.
PDB entry 3j81 coloured by chain, top view.
Source organisms:
Primary publication:
Structural changes enable start codon recognition by the eukaryotic translation initiation complex.
Hussain T, Llácer JL, Fernández IS, Munoz A, Martin-Marcos P, Savva CG, Lorsch JR, Hinnebusch AG, Ramakrishnan V
OpenAccess logo Cell 159 597-607 (2014)
PMID: 25417110
Related structures: EMD-2763

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123453 (preferred)
Entry contents:
39 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (42 distinct):
Small ribosomal subunit protein uS2 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.26 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CN12 (Residues: 1-254; Coverage: 100%)
Gene names: KLLA0E16171g, RPS0
Sequence domains: Ribosomal protein S2
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1
Small ribosomal subunit protein eS1 Chain: B
Molecule details ›
Chain: B
Length: 255 amino acids
Theoretical weight: 28.97 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWD0 (Residues: 1-255; Coverage: 100%)
Gene names: KLLA0B05060g, RPS1
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS5 Chain: C
40S ribosomal protein S3 Chain: D
40S ribosomal protein S4 Chain: E
Small ribosomal subunit protein uS7 domain-containing protein Chain: F
Small ribosomal subunit protein eS6 Chain: G
40S ribosomal protein S7 Chain: H
40S ribosomal protein S8 Chain: I
KLLA0E23673p Chain: J
Plectin/eS10 N-terminal domain-containing protein Chain: K
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: L
40S ribosomal protein S12 Chain: M
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: N
Small ribosomal subunit protein uS11 Chain: O
Rps15 Chain: P
Small ribosomal subunit protein uS9 Chain: Q
KLLA0B01474p Chain: R
KLLA0B01562p Chain: S
KLLA0A07194p Chain: T
Small ribosomal subunit protein uS10 domain-containing protein Chain: U
Small ribosomal subunit protein eS21 Chain: V
Small ribosomal subunit protein uS8 Chain: W
40S ribosomal protein S23 Chain: X
40S ribosomal protein S24 Chain: Y
40S ribosomal protein S25 Chain: Z
40S ribosomal protein S26 Chain: a
40S ribosomal protein S27 Chain: b
Small ribosomal subunit protein eS28 Chain: c
Ubiquitin-ribosomal protein eS31 fusion protein Chain: f
Asc1 Chain: g
Small ribosomal subunit protein uS14 Chain: d
40S ribosomal protein S30 Chain: e
Small ribosomal subunit protein eS32A Chain: h
Eukaryotic translation initiation factor 1A Chain: i
Eukaryotic translation initiation factor 2 subunit alpha Chain: j
Eukaryotic translation initiation factor eIF-1 Chain: m
Eukaryotic translation initiation factor 2 subunit gamma Chain: k
Eukaryotic translation initiation factor 2 subunit beta Chain: l
18S Ribosomal RNA Chain: 2
Met-tRNAi Chain: 1
Messenger RNA Chain: 3

Ligands and Environments

3 bound ligands:
Ligand MG 81 x MG
Ligand ZN 3 x ZN
Ligand MET 1 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-2763
Expression systems:
  • Escherichia coli
  • Saccharomyces cerevisiae

Quick links

Citations

24 review citations

Upstream Open Reading Frames Differentially Regulate Gene-specific Translation in the Integrated Stress Response.
Young et al. (2016)
23 more

24 mentions without citation

Quantitative studies of mRNA recruitment to the eukaryotic ribosome.
Fraser CS. (2015)
23 more