3jck

Electron Microscopy
3.5Å resolution

Structure of the yeast 26S proteasome lid sub-complex

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131727 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
26S proteasome regulatory subunit RPN3 Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 49.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40016 (Residues: 131-523; Coverage: 75%)
Gene names: RPN3, SUN2, YER021W
Sequence domains:
26S proteasome regulatory subunit RPN5 Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 51.84 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12250 (Residues: 1-445; Coverage: 100%)
Gene names: D1572, NAS5, RPN5, YDL147W
Sequence domains:
26S proteasome regulatory subunit RPN6 Chain: C
26S proteasome regulatory subunit RPN7 Chain: D
26S proteasome regulatory subunit RPN8 Chain: E
26S proteasome regulatory subunit RPN9 Chain: F
Ubiquitin carboxyl-terminal hydrolase RPN11 Chain: G
26S proteasome regulatory subunit RPN12 Chain: H
26S proteasome complex subunit SEM1 Chain: I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-6479
Expression system: Escherichia coli BL21(DE3)