3uyp

X-ray diffraction
2Å resolution

Crystal structure of the dengue virus serotype 4 envelope protein domain III in complex with the variable domains of Mab 4E11

Released:
Model geometry
Fit model/data

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-212216 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-dengue Mab 4E11 Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.25 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Envelope protein E Chain: B
Molecule details ›
Chain: B
Length: 114 amino acids
Theoretical weight: 12.57 KDa
Source organism: dengue virus type 4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YHF0 (Residues: 575-679; Coverage: 3%)
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 106.6Å b: 155.88Å c: 55.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.201
Expression system: Escherichia coli