4cr2

Electron Microscopy
7.7Å resolution

Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome

Released:

Function and Biology Details

Reactions catalysed:
Cleavage of peptide bonds with very broad specificity.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 33-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126890 (preferred)
Entry contents:
33 distinct polypeptide molecules
Macromolecules (33 distinct):
Proteasome subunit beta type-1 Chain: 1
Molecule details ›
Chain: 1
Length: 215 amino acids
Theoretical weight: 23.57 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38624 (Residues: 1-215; Coverage: 100%)
Gene names: J1407, PRE3, YJL001W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chain: 2
Molecule details ›
Chain: 2
Length: 261 amino acids
Theoretical weight: 28.3 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25043 (Residues: 1-261; Coverage: 100%)
Gene names: PUP1, YOR157C
Sequence domains:
Proteasome subunit beta type-3 Chain: 3
Proteasome subunit beta type-4 Chain: 4
Proteasome subunit beta type-5 Chain: 5
Proteasome subunit beta type-6 Chain: 6
Proteasome subunit beta type-7 Chain: 7
Proteasome subunit alpha type-1 Chain: A
Proteasome subunit alpha type-2 Chain: B
Proteasome subunit alpha type-3 Chain: C
Proteasome subunit alpha type-4 Chain: D
Proteasome subunit alpha type-5 Chain: E
Proteasome subunit alpha type-6 Chain: F
Probable proteasome subunit alpha type-7 Chain: G
26S proteasome regulatory subunit 7 homolog Chain: H
26S proteasome regulatory subunit 4 homolog Chain: I
26S proteasome regulatory subunit 8 homolog Chain: J
26S proteasome regulatory subunit 6B homolog Chain: K
26S proteasome subunit RPT4 Chain: L
26S proteasome regulatory subunit 6A Chain: M
26S proteasome regulatory subunit RPN2 Chain: N
26S proteasome regulatory subunit RPN9 Chain: O
26S proteasome regulatory subunit RPN5 Chain: P
26S proteasome regulatory subunit RPN6 Chain: Q
26S proteasome regulatory subunit RPN7 Chain: R
26S proteasome regulatory subunit RPN3 Chain: S
26S proteasome regulatory subunit RPN12 Chain: T
26S proteasome regulatory subunit RPN8 Chain: U
Ubiquitin carboxyl-terminal hydrolase RPN11 Chain: V
26S proteasome regulatory subunit RPN10 Chain: W
26S proteasome regulatory subunit RPN13 Chain: X
26S proteasome complex subunit SEM1 Chain: Y
26S proteasome regulatory subunit RPN1 Chain: Z

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-2594