4v65

Electron Microscopy
9Å resolution

Structure of the E. coli ribosome in the Pre-accommodation state

Released:
Entry authors: Devkota B, Caulfield TR, Tan R-Z, Harvey SC
Related structures: EMD-1055

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 57-mer (preferred)
PDBe Complex ID:
PDB-CPX-249863 (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
Small ribosomal subunit protein uS2 Chain: AB
Molecule details ›
Chain: AB
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS11 Chain: AC
Molecule details ›
Chain: AC
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: AD
Small ribosomal subunit protein uS13 Chain: AF
Small ribosomal subunit protein uS14 Chain: AG
Small ribosomal subunit protein uS15 Chain: AH
Small ribosomal subunit protein bS16 Chain: AI
Small ribosomal subunit protein uS17 Chain: AJ
Small ribosomal subunit protein bS18 Chain: AK
Small ribosomal subunit protein uS19 Chain: AL
Small ribosomal subunit protein bS20 Chain: AN
Small ribosomal subunit protein uS3 Chain: AO
Small ribosomal subunit protein bS21 Chain: AQ
Small ribosomal subunit protein uS4 Chain: AR
Small ribosomal subunit protein uS5 Chain: AS
Small ribosomal subunit protein bS6 Chain: AT
Small ribosomal subunit protein uS7 Chain: AU
Small ribosomal subunit protein uS8 Chain: AV
Small ribosomal subunit protein uS9 Chain: AW
Small ribosomal subunit protein uS10 Chain: AX
Large ribosomal subunit protein bL25 Chain: BC
Large ribosomal subunit protein uL14 Chain: BD
Large ribosomal subunit protein uL15 Chain: BE
Large ribosomal subunit protein uL16 Chain: BF
Large ribosomal subunit protein bL17 Chain: BG
Large ribosomal subunit protein uL18 Chain: BH
Large ribosomal subunit protein bL19 Chain: BI
Large ribosomal subunit protein bL20 Chain: BJ
Large ribosomal subunit protein bL21 Chain: BK
Large ribosomal subunit protein uL22 Chain: BL
Large ribosomal subunit protein uL23 Chain: BM
Large ribosomal subunit protein uL2 Chain: BN
Large ribosomal subunit protein uL24 Chain: BO
Large ribosomal subunit protein bL27 Chain: BP
Large ribosomal subunit protein uL29 Chain: BQ
Large ribosomal subunit protein uL30 Chain: BR
Large ribosomal subunit protein bL31 Chain: BS
Large ribosomal subunit protein bL32 Chain: BT
Large ribosomal subunit protein bL33 Chain: BU
Large ribosomal subunit protein bL34 Chain: BV
Large ribosomal subunit protein bL35 Chain: BW
Large ribosomal subunit protein bL36A Chain: BX
Large ribosomal subunit protein uL3 Chain: BY
Large ribosomal subunit protein uL1 Chain: BZ
Large ribosomal subunit protein uL4 Chain: B1
Large ribosomal subunit protein uL5 Chain: B2
Large ribosomal subunit protein uL6 Chain: B3
Large ribosomal subunit protein bL9 Chain: B4
Large ribosomal subunit protein uL11 Chain: B5
Large ribosomal subunit protein uL13 Chain: B6
A/T, P and E-site tRNAs Chains: AA, AE, AP
mRNA model Chain: AM
16S ribosomal RNA Chain: A1
5S ribosomal RNA Chain: BA
23S ribosomal RNA Chain: BB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-1055