4v6i

Electron Microscopy
8.8Å resolution

Localization of the small subunit ribosomal proteins into a 6.1 A cryo-EM map of Saccharomyces cerevisiae translating 80S ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 86-mer (preferred)
PDBe Complex ID:
PDB-CPX-249654 (preferred)
Entry contents:
77 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (83 distinct):
Small ribosomal subunit protein RACK1 Chain: Aa
Molecule details ›
Chain: Aa
Length: 319 amino acids
Theoretical weight: 34.84 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38011 (Residues: 1-319; Coverage: 100%)
Gene names: ASC1, CPC2, YM9718.15C, YMR116C
Sequence domains: WD domain, G-beta repeat
Small ribosomal subunit protein uS2A Chain: AA
Molecule details ›
Chain: AA
Length: 252 amino acids
Theoretical weight: 28.05 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32905 (Residues: 1-252; Coverage: 100%)
Gene names: NAB1, NAB1A, RPS0A, YGR214W, YST1
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: AB
40S ribosomal protein rpS4 (S4e) Chain: AD
Small ribosomal subunit protein uS4A Chain: AC
Small ribosomal subunit protein uS5 Chain: AE
40S ribosomal protein rpS7 (S7e) Chain: AG
Small ribosomal subunit protein uS7 Chain: AF
Small ribosomal subunit protein uS8A Chain: AH
40S ribosomal protein rpS16 (S9p) Chain: AI
Small ribosomal subunit protein uS10 Chain: AJ
Small ribosomal subunit protein uS11A Chain: AK
40S ribosomal protein rpS23 (S12p) Chain: AL
40S ribosomal protein rpS18 (S13p) Chain: AM
Small ribosomal subunit protein uS14A Chain: AN
Small ribosomal subunit protein uS15 Chain: AO
Small ribosomal subunit protein eS17A Chain: AQ
40S ribosomal protein rpS11 (S17p) Chain: AP
Small ribosomal subunit protein uS19 Chain: AR
Small ribosomal subunit protein eS19A Chain: AS
Small ribosomal subunit protein eS21A Chain: AT
Small ribosomal subunit protein eS25A Chain: AV
40S ribosomal protein rpS26 (S26e) Chain: AW
Small ribosomal subunit protein eS27A Chain: AX
Small ribosomal subunit protein eS28A Chain: AY
40S ribosomal protein rpS30 (S30e) Chain: AZ
Unknown 40S ribosomal protein XS1 Chain: Ab
Unknown 40S ribosomal protein XS2 Chain: Ac
40S ribosomal protein S24 Chain: AU
60S ribosomal protein rpL1 (L1p) Chain: BA
60S ribosomal protein rpL2 (L2p) Chain: BB
Large ribosomal subunit protein uL3 Chain: BC
Large ribosomal subunit protein uL4A Chain: BD
Large ribosomal subunit protein uL5A Chain: BE
Large ribosomal subunit protein eL6A Chain: BG
Large ribosomal subunit protein uL6A Chain: BF
Large ribosomal subunit protein eL8A Chain: BH
Large ribosomal subunit protein uL10 Chain: Bs
60S ribosomal protein rpL12 (L11p) Chain: BJ
Large ribosomal subunit protein uL13A Chain: BK
Large ribosomal subunit protein eL14A Chain: BN
60S ribosomal protein rpL23 (L14p) Chain: BM
Large ribosomal subunit protein eL15A Chain: BP
Large ribosomal subunit protein uL15 Chain: BO
60S ribosomal protein rpL18 (L18e) Chain: BR
60S ribosomal protein rpL19 (L19e) Chain: BT
Large ribosomal subunit protein eL21A Chain: BU
Large ribosomal subunit protein eL22A Chain: BW
Large ribosomal subunit protein uL22A Chain: BV
Large ribosomal subunit protein uL23 Chain: BX
Large ribosomal subunit protein eL24A Chain: BZ
Large ribosomal subunit protein uL24A Chain: BY
Large ribosomal subunit protein eL27A Chain: Ba
Large ribosomal subunit protein eL29 Chain: Bd
60S ribosomal protein rpL35 (L29p) Chain: Bc
Large ribosomal subunit protein eL30 Chain: Bf
Large ribosomal subunit protein uL30A Chain: Be
Large ribosomal subunit protein eL31A Chain: Bg
Large ribosomal subunit protein eL32 Chain: Bh
Large ribosomal subunit protein eL34A Chain: Bi
Large ribosomal subunit protein eL33A Chain: Bj
Large ribosomal subunit protein eL36A Chain: Bk
60S ribosomal protein rpL43 (L37ae) Chain: Bm
Large ribosomal subunit protein eL37A Chain: Bl
Large ribosomal subunit protein eL38 Chain: Bn
Large ribosomal subunit protein eL39 Chain: Bo
Large ribosomal subunit protein eL40A Chain: Bp
60S ribosomal protein rpL41 (L41e) Chain: Bq
60S ribosomal protein rpL42 (L44e) Chain: Br
Unknown protein Chains: Bx, By
Unknown protein Chain: Bz
Large ribosomal subunit protein P1A Chains: Bt, Bu
Large ribosomal subunit protein P2A Chains: Bv, Bw
Large ribosomal subunit protein uL18 Chain: BQ
60S ribosomal protein rpL13 (L13e) Chain: BL
Large ribosomal subunit protein eL20 Chain: BS
Large ribosomal subunit protein uL16 Chain: BI
18S Ribosomal RNA Chain: CA
P-SITE TRNA ASP Chain: CB
MRNA, RNA (5'-R(P*AP*AP*AP*AP*GP*AP*CP*UP*UP*CP*A)-3') Chain: CC
25S ribosomal RNA Chain: DA
5.8S ribosomal RNA Chain: DB
5S ribosomal RNA Chain: DC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.8Å
Relevant EMDB volumes: EMD-1669