› 4ffs

X-ray diffraction
1.9Å resolution

Crystal structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Helicobacter pylori with butyl-thio-DADMe-Immucillin-A

Released:
DOI: 10.2210/pdb4ffs/pdb
Model geometry
Fit model/data
PDB entry 4ffs coloured by chain, front view.
PDB entry 4ffs coloured by chain, side view.
PDB entry 4ffs coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
A picomolar transition state analogue inhibitor of MTAN as a specific antibiotic for Helicobacter pylori.
Wang S, Haapalainen AM, Yan F, Du Q, Tyler PC, Evans GB, Rinaldo-Matthis A, Brown RL, Norris GE, Almo SC, Schramm VL
Biochemistry 51 6892-4 (2012)
PMID: 22891633

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 26.29 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZMY2 (Residues: 1-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand BIG 1 x BIG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I432
Unit cell:
a: 157.732Å b: 157.732Å c: 157.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Defining the metabolic requirements for the growth and colonization capacity of Campylobacter jejuni.
Hofreuter D. (2014)
5 more

3 mentions without citation

A picomolar transition state analogue inhibitor of MTAN as a specific antibiotic for Helicobacter pylori.
Wang et al. (2012)
2 more