4j6e

X-ray diffraction
2.52Å resolution

Structure of LPXI D225A Mutant

Released:
Model geometry
Fit model/data
Primary publication:
LpxI structures reveal how a lipid A precursor is synthesized.
Nat Struct Mol Biol 19 1132-8 (2012)
PMID: 23042606

Function and Biology Details

Reaction catalysed:
A UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + H(2)O = a lipid X + UMP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-101819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2,3-diacylglucosamine pyrophosphatase LpxI Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 29.96 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3C8Q1 (Residues: 1-280; Coverage: 100%)
Gene names: CCNA_01987, lpxI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6122
Unit cell:
a: 107.297Å b: 107.297Å c: 81.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.221 0.286
Expression system: Escherichia coli