› 4qnv

X-ray diffraction
2.64Å resolution

Crystal structure of Cx-SAM bound CmoB from E. coli in P6122

Released:
DOI: 10.2210/pdb4qnv/pdb
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PDB entry 4qnv coloured by chain, front view.
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PDB entry 4qnv coloured by chain, top view.
Source organism: Escherichia coli APEC O1
Primary publication:
Determinants of the CmoB carboxymethyl transferase utilized for selective tRNA wobble modification.
Kim J, Xiao H, Koh J, Wang Y, Bonanno JB, Thomas K, Babbitt PC, Brown S, Lee YS, Almo SC
OpenAccess logo Nucleic Acids Res 43 4602-13 (2015)
PMID: 25855808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA U34 carboxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 37.05 KDa
Source organism: Escherichia coli APEC O1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1AC32 (Residues: 1-323; Coverage: 100%)
Gene names: APECO1_921, Ecok1_17280, cmoB
Sequence domains: Protein of unknown function (DUF1698)

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GEK 2 x GEK
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 82.134Å b: 82.134Å c: 432.516Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Recent advances in methyltransferase biocatalysis.
Bennett et al. (2017)
1 more

3 mentions without citation

S-Adenosylmethionine: more than just a methyl donor.
Lee et al. (2023)
2 more

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