5bxt

X-ray diffraction
1.8Å resolution

LNBase in complex with LNB-NHAcAUS

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
Beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H(2)O = beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lacto-N-biosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 644 amino acids
Theoretical weight: 71.89 KDa
Source organism: Bifidobacterium bifidum JCM 1254
Expression system: Escherichia coli
UniProt:
  • Canonical: B3TLD6 (Residues: 41-663; Coverage: 58%)
Gene name: lnbB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21212
Unit cell:
a: 116.985Å b: 131.513Å c: 104.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.209
Expression system: Escherichia coli