5j88

X-ray diffraction
3.32Å resolution

Structure of the E coli 70S ribosome with the U1060A mutation in 16S rRNA

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero 53-mer (preferred)
hetero 52-mer
PDBe Complex ID:
PDB-CPX-249817 (preferred)
Entry contents:
51 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (55 distinct):
Small ribosomal subunit protein uS2 Chains: AB, BB
Molecule details ›
Chains: AB, BB
Length: 224 amino acids
Theoretical weight: 24.97 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V0 (Residues: 4-227; Coverage: 93%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chains: AC, BC
Molecule details ›
Chains: AC, BC
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V3 (Residues: 2-207; Coverage: 88%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chains: AD, BD
Small ribosomal subunit protein uS5 Chains: AE, BE
Small ribosomal subunit protein bS6, non-modified isoform Chains: AF, BF
Small ribosomal subunit protein uS7 Chains: AG, BG
Small ribosomal subunit protein uS8 Chains: AH, BH
Small ribosomal subunit protein uS9 Chains: AI, BI
Small ribosomal subunit protein uS10 Chains: AJ, BJ
Small ribosomal subunit protein uS11 Chains: AK, BK
Small ribosomal subunit protein uS12 Chains: AL, BL
Small ribosomal subunit protein uS13 Chains: AM, BM
Small ribosomal subunit protein uS14 Chains: AN, BN
Small ribosomal subunit protein uS15 Chains: AO, BO
Small ribosomal subunit protein bS16 Chains: AP, BP
Small ribosomal subunit protein uS17 Chains: AQ, BQ
Small ribosomal subunit protein bS18 Chains: AR, BR
Small ribosomal subunit protein uS19 Chains: AS, BS
Small ribosomal subunit protein bS20 Chains: AT, BT
Small ribosomal subunit protein bS21 Chains: AU, BU
Large ribosomal subunit protein bL32 Chains: C1, D1
Large ribosomal subunit protein bL33 Chains: C2, D2
Large ribosomal subunit protein bL34 Chains: C3, D3
Large ribosomal subunit protein bL35 Chains: C4, D4
Large ribosomal subunit protein bL36A Chains: C5, D5
Large ribosomal subunit protein uL30 Chains: C0, D0
Large ribosomal subunit protein uL2 Chains: CC, DC
Large ribosomal subunit protein uL3 Chain: CD
Large ribosomal subunit protein uL3 Chain: DD
Large ribosomal subunit protein uL4 Chains: CE, DE
Large ribosomal subunit protein uL5 Chains: CF, DF
Large ribosomal subunit protein uL6 Chains: CG, DG
Large ribosomal subunit protein bL9 Chains: CH, DH
Large ribosomal subunit protein uL11 Chains: CJ, DJ
Large ribosomal subunit protein uL13 Chains: CK, DK
Large ribosomal subunit protein uL14 Chains: CL, DL
Large ribosomal subunit protein uL15 Chains: CM, DM
Large ribosomal subunit protein uL16 Chains: CN, DN
Large ribosomal subunit protein bL17 Chains: CO, DO
Large ribosomal subunit protein uL18 Chains: CP, DP
Large ribosomal subunit protein bL19 Chains: CQ, DQ
Large ribosomal subunit protein bL20 Chains: CR, DR
Large ribosomal subunit protein bL21 Chains: CS, DS
Large ribosomal subunit protein uL22 Chains: CT, DT
Large ribosomal subunit protein uL23 Chains: CU, DU
Large ribosomal subunit protein uL24 Chains: CV, DV
Large ribosomal subunit protein bL25 Chains: CW, DW
Large ribosomal subunit protein bL27 Chains: CX, DX
Large ribosomal subunit protein bL28 Chains: CY, DY
Large ribosomal subunit protein uL29 Chains: CZ, DZ
Large ribosomal subunit protein uL10 Chain: DI
16S ribosomal RNA Chains: AA, BA
5S ribosomal RNA Chains: CB, DB
23S ribosomal RNA Chain: CA
23S ribosomal RNA Chain: DA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 211.55Å b: 433.65Å c: 622.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.219