5jut

Electron Microscopy
4Å resolution

Saccharomyces cerevisiae 80S ribosome bound with elongation factor eEF2-GDP-sordarin and Taura Syndrome Virus IRES, Structure IV (almost non-rotated 40S subunit)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 83-mer (preferred)
PDBe Complex ID:
PDB-CPX-249658 (preferred)
Entry contents:
78 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (83 distinct):
Large ribosomal subunit protein uL1A Chain: E
Molecule details ›
Chain: E
Length: 217 amino acids
Theoretical weight: 24.52 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX43 (Residues: 1-217; Coverage: 100%)
Gene names: RPL1A, SSM1, SSM1A, YPL220W
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL2A Chain: F
Molecule details ›
Chain: F
Length: 254 amino acids
Theoretical weight: 27.46 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX45 (Residues: 1-254; Coverage: 100%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains:
Large ribosomal subunit protein uL3 Chain: G
Large ribosomal subunit protein uL4A Chain: H
Large ribosomal subunit protein uL18 Chain: I
Large ribosomal subunit protein eL6A Chain: J
Large ribosomal subunit protein uL30A Chain: K
Large ribosomal subunit protein eL8A Chain: L
Large ribosomal subunit protein uL6A Chain: M
Large ribosomal subunit protein uL16 Chain: N
Large ribosomal subunit protein uL5A Chain: O
Large ribosomal subunit protein uL11A Chain: P
Large ribosomal subunit protein eL13A Chain: Q
Large ribosomal subunit protein eL14A Chain: R
Large ribosomal subunit protein eL15A Chain: S
Large ribosomal subunit protein uL13A Chain: T
Large ribosomal subunit protein uL22A Chain: U
Large ribosomal subunit protein eL18A Chain: V
Large ribosomal subunit protein eL19A Chain: W
Large ribosomal subunit protein eL20A Chain: X
Large ribosomal subunit protein eL21A Chain: Y
Large ribosomal subunit protein eL22A Chain: Z
Large ribosomal subunit protein uL14A Chain: AA
Large ribosomal subunit protein eL24A Chain: BA
Large ribosomal subunit protein uL23 Chain: CA
Large ribosomal subunit protein uL24A Chain: DA
Large ribosomal subunit protein eL27A Chain: EA
Large ribosomal subunit protein uL15 Chain: FA
Large ribosomal subunit protein eL29 Chain: GA
Large ribosomal subunit protein eL30 Chain: HA
Large ribosomal subunit protein eL31A Chain: IA
Large ribosomal subunit protein eL32 Chain: JA
Large ribosomal subunit protein eL33A Chain: KA
Large ribosomal subunit protein eL34A Chain: LA
Large ribosomal subunit protein uL29A Chain: MA
Large ribosomal subunit protein eL36A Chain: NA
Large ribosomal subunit protein eL37A Chain: OA
Large ribosomal subunit protein eL38 Chain: PA
Large ribosomal subunit protein eL39 Chain: QA
Ubiquitin-ribosomal protein eL40A fusion protein Chain: RA
Small ribosomal subunit protein eS32A Chain: SA
Large ribosomal subunit protein eL42A Chain: TA
Large ribosomal subunit protein eL43A Chain: UA
Large ribosomal subunit protein uL10 Chain: VA
Small ribosomal subunit protein RACK1 Chain: WA
Small ribosomal subunit protein uS2A Chain: XA
Small ribosomal subunit protein eS1A Chain: YA
Small ribosomal subunit protein uS5 Chain: ZA
Small ribosomal subunit protein uS3 Chain: AB
Small ribosomal subunit protein eS4A Chain: BB
Small ribosomal subunit protein uS7 Chain: CB
Small ribosomal subunit protein eS6A Chain: DB
Small ribosomal subunit protein eS7A Chain: EB
Small ribosomal subunit protein eS8A Chain: FB
Small ribosomal subunit protein uS4A Chain: GB
Small ribosomal subunit protein eS10A Chain: HB
Small ribosomal subunit protein uS17A Chain: IB
Small ribosomal subunit protein eS12 Chain: JB
Small ribosomal subunit protein uS15 Chain: KB
Small ribosomal subunit protein uS11A Chain: LB
Small ribosomal subunit protein uS19 Chain: MB
Small ribosomal subunit protein uS9A Chain: NB
Small ribosomal subunit protein eS17A Chain: OB
Small ribosomal subunit protein uS13A Chain: PB
Small ribosomal subunit protein eS19A Chain: QB
Small ribosomal subunit protein uS10 Chain: RB
Small ribosomal subunit protein eS21A Chain: SB
Small ribosomal subunit protein uS8A Chain: TB
Small ribosomal subunit protein uS12A Chain: UB
Small ribosomal subunit protein eS24A Chain: VB
Small ribosomal subunit protein eS25A Chain: WB
Small ribosomal subunit protein eS26A Chain: XB
Small ribosomal subunit protein eS27A Chain: YB
Small ribosomal subunit protein eS28A Chain: ZB
Small ribosomal subunit protein uS14A Chain: AC
Small ribosomal subunit protein eS30A Chain: BC
Ubiquitin-ribosomal protein eS31 fusion protein Chain: CC
Elongation factor 2 Chain: DC
18S Ribosomal RNA Chain: A
25S ribosomal RNA Chain: B
5.8S ribosomal RNA Chain: C
5S ribosomal RNA Chain: D
IRES Chain: EC

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-6646, EMD-6652