5kcr

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Avilamycin C, mRNA and P-site tRNA at 3.6A resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-135571 (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein uL2 Chain: 1D
Molecule details ›
Chain: 1D
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: 1E
Molecule details ›
Chain: 1E
Length: 209 amino acids
Theoretical weight: 22.29 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: 1F
Large ribosomal subunit protein uL5 Chain: 1G
Large ribosomal subunit protein uL6 Chain: 1H
Large ribosomal subunit protein bL9 Chain: 1I
Large ribosomal subunit protein uL10 Chain: 1J
Large ribosomal subunit protein uL11 Chain: 1K
Large ribosomal subunit protein uL13 Chain: 1N
Large ribosomal subunit protein uL14 Chain: 1O
Large ribosomal subunit protein uL15 Chain: 1P
Large ribosomal subunit protein uL16 Chain: 1Q
Large ribosomal subunit protein bL17 Chain: 1R
Large ribosomal subunit protein uL18 Chain: 1S
Large ribosomal subunit protein bL19 Chain: 1T
Large ribosomal subunit protein bL20 Chain: 1U
Large ribosomal subunit protein bL21 Chain: 1V
Large ribosomal subunit protein uL22 Chain: 1W
Large ribosomal subunit protein uL23 Chain: 1X
Large ribosomal subunit protein uL24 Chain: 1Y
Large ribosomal subunit protein bL25 Chain: 1Z
Large ribosomal subunit protein bL27 Chain: 10
Large ribosomal subunit protein bL28 Chain: 11
Large ribosomal subunit protein uL29 Chain: 12
Large ribosomal subunit protein uL30 Chain: 13
Large ribosomal subunit protein bL32 Chain: 15
Large ribosomal subunit protein bL33 Chain: 16
Large ribosomal subunit protein bL34 Chain: 17
Large ribosomal subunit protein bL35 Chain: 18
Large ribosomal subunit protein bL36A Chain: 19
Small ribosomal subunit protein uS2 Chain: 1b
Small ribosomal subunit protein uS3 Chain: 1c
Small ribosomal subunit protein uS4 Chain: 1d
Small ribosomal subunit protein uS5 Chain: 1e
Small ribosomal subunit protein bS6 Chain: 1f
Small ribosomal subunit protein uS7 Chain: 1g
Small ribosomal subunit protein uS8 Chain: 1h
Small ribosomal subunit protein uS9 Chain: 1i
Small ribosomal subunit protein uS10 Chain: 1j
Small ribosomal subunit protein uS11 Chain: 1k
Small ribosomal subunit protein uS12 Chain: 1l
Small ribosomal subunit protein uS13 Chain: 1m
Small ribosomal subunit protein uS14 Chain: 1n
Small ribosomal subunit protein uS15 Chain: 1o
Small ribosomal subunit protein bS16 Chain: 1p
Small ribosomal subunit protein uS17 Chain: 1q
Small ribosomal subunit protein bS18 Chain: 1r
Small ribosomal subunit protein uS19 Chain: 1s
Small ribosomal subunit protein bS20 Chain: 1t
Small ribosomal subunit protein bS21 Chain: 1u
23S ribosomal RNA Chain: 1A
5S ribosomal RNA Chain: 1B
16S ribosomal RNA Chain: 1a
Messenger RNA Chain: 1v
P-site tRNA Chain: 1x

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8237