› 5kvi

X-ray diffraction
2Å resolution

Crystal structure of monomeric human apoptosis-inducing factor with E413A/R422A/R430A mutations

Released:
DOI: 10.2210/pdb5kvi/pdb
PDB entry 5kvi coloured by chain, front view.
PDB entry 5kvi coloured by chain, side view.
PDB entry 5kvi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.
Brosey CA, Ho C, Long WZ, Singh S, Burnett K, Hura GL, Nix JC, Bowman GR, Ellenberger T, Tainer JA
Structure 24 2067-2079 (2016)
PMID: 27818101

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 59.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95831 (Residues: 78-613; Coverage: 87%)
  • Best match: O95831-4 (Residues: 78-322)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FAD 1 x FAD
Ligand EPE 1 x EPE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 64.623Å b: 81.802Å c: 108.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
Syed et al. (2018)
6 more

2 mentions without citation

Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.
Brosey et al. (2016)
1 more