5nco

Electron Microscopy
4.8Å resolution

Quaternary complex between SRP, SR, and SecYEG bound to the translating ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 40-mer (preferred)
PDBe Complex ID:
PDB-CPX-249953 (preferred)
Entry contents:
36 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (40 distinct):
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein bL9 Chain: H
Large ribosomal subunit protein uL10 Chain: I
Large ribosomal subunit protein uL11 Chain: J
Large ribosomal subunit protein uL13 Chain: K
Large ribosomal subunit protein uL14 Chain: L
Large ribosomal subunit protein uL15 Chain: M
Large ribosomal subunit protein uL16 Chain: N
Large ribosomal subunit protein bL17 Chain: O
Large ribosomal subunit protein uL18 Chain: P
Large ribosomal subunit protein bL19 Chain: Q
Large ribosomal subunit protein bL20 Chain: R
Large ribosomal subunit protein bL21 Chain: S
Large ribosomal subunit protein uL22 Chain: T
Large ribosomal subunit protein uL23 Chain: U
Large ribosomal subunit protein uL24 Chain: V
Large ribosomal subunit protein bL25 Chain: W
Large ribosomal subunit protein bL27 Chain: X
Large ribosomal subunit protein bL28 Chain: Y
Large ribosomal subunit protein uL29 Chain: Z
Large ribosomal subunit protein uL30 Chain: a
Large ribosomal subunit protein bL32 Chain: b
Large ribosomal subunit protein bL33 Chain: c
Large ribosomal subunit protein bL34 Chain: d
Large ribosomal subunit protein bL35 Chain: e
Large ribosomal subunit protein bL36A Chain: f
Protein translocase subunit SecY Chain: g
Protein translocase subunit SecE Chain: h
Signal recognition particle protein Chain: i
Protein-export membrane protein SecG Chain: j
Signal sequence (1A9L) Chain: k
Signal recognition particle receptor FtsY Chain: l
4.5S SRP RNA (Ffs) Chain: 1
P-site tRNA-CCA end Chain: 2
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-3617
Expression system: Escherichia coli BL21