5u9g

Electron Microscopy
3.2Å resolution

3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-249778 (preferred)
Entry contents:
54 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (59 distinct):
Alternative ribosome-rescue factor A Chain: Y
Molecule details ›
Chain: Y
Length: 72 amino acids
Theoretical weight: 8.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P36675 (Residues: 1-72; Coverage: 100%)
Gene names: JW3253, arfA, b4550, yhdL
Sequence domains: Alternative ribosome-rescue factor A
Large ribosomal subunit protein uL1 Chain: 03
Molecule details ›
Chain: 03
Length: 234 amino acids
Theoretical weight: 24.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 1-234; Coverage: 100%)
Gene names: JW3947, b3984, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL2 Chain: 04
Large ribosomal subunit protein uL3 Chain: 05
Large ribosomal subunit protein uL4 Chain: 06
Large ribosomal subunit protein uL5 Chain: 07
Large ribosomal subunit protein uL6 Chain: 08
Large ribosomal subunit protein bL9 Chain: 09
Large ribosomal subunit protein uL10 Chain: 10
Large ribosomal subunit protein uL11 Chain: 11
Large ribosomal subunit protein uL13 Chain: 12
Large ribosomal subunit protein uL14 Chain: 13
Large ribosomal subunit protein uL15 Chain: 14
Large ribosomal subunit protein uL16 Chain: 15
Large ribosomal subunit protein bL17 Chain: 16
Large ribosomal subunit protein uL18 Chain: 17
Large ribosomal subunit protein bL19 Chain: 18
Large ribosomal subunit protein bL20 Chain: 19
Large ribosomal subunit protein bL21 Chain: 20
Large ribosomal subunit protein uL22 Chain: 21
Large ribosomal subunit protein uL23 Chain: 22
Large ribosomal subunit protein uL24 Chain: 23
Large ribosomal subunit protein bL25 Chain: 24
Large ribosomal subunit protein bL27 Chain: 25
Large ribosomal subunit protein bL28 Chain: 26
Large ribosomal subunit protein uL29 Chain: 27
Large ribosomal subunit protein uL30 Chain: 28
Large ribosomal subunit protein bL31 Chain: 29
Large ribosomal subunit protein bL32 Chain: 30
Large ribosomal subunit protein bL33 Chain: 31
Large ribosomal subunit protein bL34 Chain: 32
Large ribosomal subunit protein bL35 Chain: 33
Large ribosomal subunit protein bL36A Chain: 34
Peptide chain release factor RF2 Chain: Z
Small ribosomal subunit protein uS2 Chain: B
Small ribosomal subunit protein uS3 Chain: C
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bS21 Chain: U
16S ribosomal RNA Chain: A
23S ribosomal RNA Chain: 01
5S ribosomal RNA Chain: 02
fMet-tRNA (P- and E-site) Chains: W, X
truncated mRNA Chain: V

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-8522
Expression system: Escherichia coli K-12