5wsv

X-ray diffraction
2.33Å resolution

Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-143934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 151 amino acids
Theoretical weight: 16.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-147; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Unconventional myosin-VIIa Chains: B, D
Molecule details ›
Chains: B, D
Length: 47 amino acids
Theoretical weight: 5.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P97479 (Residues: 828-870; Coverage: 2%)
Gene names: Myo7, Myo7a
Sequence domains: IQ calmodulin-binding motif

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 38.683Å b: 89.755Å c: 45.724Å
α: 90° β: 106.03° γ: 90°
R-values:
R R work R free
0.23 0.228 0.262
Expression system: Escherichia coli