5ylz

Electron Microscopy
3.6Å resolution

Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom

Released:
Primary publication:
Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae.
Cell 171 1589-1598.e8 (2017)
PMID: 29153833
Related structures: EMD-6839

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 43-mer (preferred)
PDBe Complex ID:
PDB-CPX-250230 (preferred)
Entry contents:
29 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (33 distinct):
Pre-mRNA-splicing factor 8 Chain: A
Pre-mRNA-splicing factor SNU114 Chain: C
Molecule details ›
Chain: C
Length: 1008 amino acids
Theoretical weight: 114.17 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P36048 (Residues: 1-1008; Coverage: 100%)
Gene names: GIN10, SNU114, YKL173W, YKL637
Sequence domains:
Pre-mRNA-splicing factor SNT309 Chain: G
Pre-mRNA-splicing factor SYF1 Chain: H
Pre-mRNA-splicing factor CLF1 Chain: I
Pre-mRNA-splicing factor CEF1 Chain: J
Pre-mRNA-splicing factor SYF2 Chain: K
Pre-mRNA-splicing factor BUD31 Chain: L
Pre-mRNA-processing factor 17 Chain: T
Pre-mRNA-splicing factor SLT11 Chain: M
Pre-mRNA-splicing factor CWC2 Chain: N
Pre-mRNA-splicing factor PRP46 Chain: O
Pre-mRNA-splicing factor CWC15 Chain: P
Pre-mRNA-processing protein 45 Chain: Q
Pre-mRNA-splicing factor CWC21 Chain: R
Pre-mRNA-splicing factor CWC22 Chain: S
Pre-mRNA-splicing factor 18 Chain: U
Pre-mRNA-splicing factor SLU7 Chain: V
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 Chain: W
Small nuclear ribonucleoprotein-associated protein B Chains: a, h
Small nuclear ribonucleoprotein E Chains: b, i
Small nuclear ribonucleoprotein F Chains: c, j
Small nuclear ribonucleoprotein G Chains: d, k
Small nuclear ribonucleoprotein Sm D3 Chains: e, l
Small nuclear ribonucleoprotein Sm D1 Chains: f, m
Small nuclear ribonucleoprotein Sm D2 Chains: g, n
U2 small nuclear ribonucleoprotein A' Chain: o
U2 small nuclear ribonucleoprotein B'' Chain: p
Pre-mRNA-processing factor 19 Chains: q, r, s, t
U5 snRNA Chain: B
U6 snRNA Chain: D
mRNA/intron lariat Chain: E
U2 snRNA Chain: F

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-6839