6gxm

Electron Microscopy
3.8Å resolution

Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State II)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-249796 (preferred)
Entry contents:
53 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (58 distinct):
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein bL9 Chain: H
Large ribosomal subunit protein uL11 Chain: I
Large ribosomal subunit protein uL13 Chain: J
Large ribosomal subunit protein uL14 Chain: K
Large ribosomal subunit protein uL15 Chain: L
Large ribosomal subunit protein uL16 Chain: M
Large ribosomal subunit protein bL17 Chain: N
Large ribosomal subunit protein uL18 Chain: O
Large ribosomal subunit protein bL19 Chain: P
Large ribosomal subunit protein bL20 Chain: Q
Large ribosomal subunit protein bL21 Chain: R
Large ribosomal subunit protein uL22 Chain: S
Large ribosomal subunit protein uL23 Chain: T
Large ribosomal subunit protein uL24 Chain: U
Large ribosomal subunit protein bL25 Chain: V
Large ribosomal subunit protein bL27 Chain: W
Large ribosomal subunit protein bL28 Chain: X
Large ribosomal subunit protein uL29 Chain: Y
Large ribosomal subunit protein uL30 Chain: Z
Large ribosomal subunit protein bL32 Chain: 0
Large ribosomal subunit protein bL33 Chain: 1
Large ribosomal subunit protein bL34 Chain: 2
Large ribosomal subunit protein bL35 Chain: 3
Large ribosomal subunit protein bL36A Chain: 4
Large ribosomal subunit protein uL10 Chain: 5
Small ribosomal subunit protein uS2 Chain: b
Small ribosomal subunit protein uS3 Chain: c
Small ribosomal subunit protein uS4 Chain: d
Small ribosomal subunit protein uS5 Chain: e
Small ribosomal subunit protein bS6, non-modified isoform Chain: f
Small ribosomal subunit protein uS7 Chain: g
Small ribosomal subunit protein uS8 Chain: h
Small ribosomal subunit protein uS9 Chain: i
Small ribosomal subunit protein uS10 Chain: j
Small ribosomal subunit protein uS11 Chain: k
Small ribosomal subunit protein uS12 Chain: l
Small ribosomal subunit protein uS13 Chain: m
Small ribosomal subunit protein uS14 Chain: n
Small ribosomal subunit protein uS15 Chain: o
Small ribosomal subunit protein bS16 Chain: p
Small ribosomal subunit protein uS17 Chain: q
Small ribosomal subunit protein bS18 Chain: r
Small ribosomal subunit protein uS19 Chain: s
Small ribosomal subunit protein bS20 Chain: t
Small ribosomal subunit protein bS21 Chain: u
Peptide chain release factor RF1 Chain: v
Peptide chain release factor RF3 Chain: w
Apidaecin Chain: z
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B
Messenger RNA Chain: 7
16S ribosomal RNA Chain: a
fMet-tRNA Chain: x

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0080
Expression system: Not provided