6lnc

Electron Microscopy
3.21Å resolution

CryoEM structure of Cascade-TniQ complex

Released:
Source organism: Vibrio cholerae
Related structures: EMD-0930

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-167030 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated protein Cas7 Chains: B, C, D, E, F, G
Molecule details ›
Chains: B, C, D, E, F, G
Length: 354 amino acids
Theoretical weight: 40.03 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
CRISPR-associated protein Cas8 Chain: H
Molecule details ›
Chain: H
Length: 640 amino acids
Theoretical weight: 72.29 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
CRISPR-associated protein Cas6 Chain: A
transposition protein TniQ Chains: I, J
CRISPR RNA (60-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.21Å
Relevant EMDB volumes: EMD-0930
Expression system: Escherichia coli BL21(DE3)