6msh

Electron Microscopy
3.6Å resolution

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
PDBe Complex ID:
PDB-CPX-126180 (preferred)
Entry contents:
33 distinct polypeptide molecules
Macromolecules (33 distinct):
26S proteasome non-ATPase regulatory subunit 1 Chain: U
Molecule details ›
Chain: U
Length: 953 amino acids
Theoretical weight: 105.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99460 (Residues: 1-953; Coverage: 100%)
Gene name: PSMD1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 3 Chain: V
Molecule details ›
Chain: V
Length: 533 amino acids
Theoretical weight: 60.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43242 (Residues: 2-534; Coverage: 100%)
Gene name: PSMD3
Sequence domains:
26S proteasome non-ATPase regulatory subunit 12 Chain: W
26S proteasome non-ATPase regulatory subunit 11 Chain: X
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
26S proteasome non-ATPase regulatory subunit 13 Chain: a
26S proteasome non-ATPase regulatory subunit 4 Chain: b
26S proteasome non-ATPase regulatory subunit 14 Chain: c
26S proteasome non-ATPase regulatory subunit 8 Chain: d
26S proteasome complex subunit SEM1 Chain: e
26S proteasome non-ATPase regulatory subunit 2 Chain: f
26S proteasome regulatory subunit 7 Chain: A
26S proteasome regulatory subunit 4 Chain: B
26S proteasome regulatory subunit 8 Chain: C
26S proteasome regulatory subunit 6B Chain: D
26S proteasome regulatory subunit 10B Chain: E
26S proteasome regulatory subunit 6A Chain: F
substrate Chain: v
Proteasome subunit alpha type-6 Chains: G, g
Proteasome subunit alpha type-2 Chains: H, h
Proteasome subunit alpha type-4 Chains: I, i
Proteasome subunit alpha type-7 Chains: J, j
Proteasome subunit alpha type-5 Chains: K, k
Proteasome subunit alpha type-1 Chains: L, l
Proteasome subunit alpha type-3 Chains: M, m
Proteasome subunit beta type-6 Chains: N, n
Proteasome subunit beta type-7 Chains: O, o
Proteasome subunit beta type-3 Chains: P, p
Proteasome subunit beta type-2 Chains: Q, q
Proteasome subunit beta type-5 Chains: R, r
Proteasome subunit beta type-1 Chains: S, s
Proteasome subunit beta type-4 Chains: T, t

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-9220
Expression systems:
  • Homo sapiens
  • Escherichia coli K-12