6qhp

X-ray diffraction
1.8Å resolution

Time resolved structural analysis of the full turnover of an enzyme - 2256 ms covalent intermediate 1

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
Haloacetate + H(2)O = glycolate + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-564190 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoroacetate dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.13 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NAM1 (Residues: 1-302; Coverage: 100%)
Gene name: RPA1163
Sequence domains: alpha/beta hydrolase fold
Fluoroacetate dehalogenase Chain: B
Molecule details ›
Chain: B
Length: 306 amino acids
Theoretical weight: 34.07 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NAM1 (Residues: 1-302; Coverage: 100%)
Gene name: RPA1163
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 41.4Å b: 78.67Å c: 83.59Å
α: 90° β: 102.73° γ: 90°
R-values:
R R work R free
0.22 0.218 0.266
Expression system: Escherichia coli