6s47

Electron Microscopy
3.28Å resolution

Saccharomyces cerevisiae 80S ribosome bound with ABCF protein New1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 79-mer (preferred)
PDBe Complex ID:
PDB-CPX-103558 (preferred)
Entry contents:
75 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (79 distinct):
Large ribosomal subunit protein uL2A Chain: AD
Molecule details ›
Chain: AD
Length: 252 amino acids
Theoretical weight: 27.22 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX45 (Residues: 2-253; Coverage: 99%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains:
Large ribosomal subunit protein uL3 Chain: AE
Molecule details ›
Chain: AE
Length: 386 amino acids
Theoretical weight: 43.72 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14126 (Residues: 2-387; Coverage: 100%)
Gene names: MAK8, RPL3, TCM1, YOR063W, YOR29-14
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4A Chain: AF
Large ribosomal subunit protein uL18 Chain: AG
Large ribosomal subunit protein eL6A Chain: AH
Large ribosomal subunit protein uL30A Chain: AI
Large ribosomal subunit protein eL8A Chain: AJ
Large ribosomal subunit protein uL6A Chain: AK
Large ribosomal subunit protein uL16 Chain: AL
Large ribosomal subunit protein uL5B Chain: AM
Large ribosomal subunit protein eL13A Chain: AN
Large ribosomal subunit protein eL14A Chain: AO
Large ribosomal subunit protein eL15A Chain: AP
Large ribosomal subunit protein uL13A Chain: AQ
Large ribosomal subunit protein uL22A Chain: AR
Large ribosomal subunit protein eL18A Chain: AS
Large ribosomal subunit protein eL19A Chain: AT
Large ribosomal subunit protein eL20A Chain: AU
Large ribosomal subunit protein eL21A Chain: AV
Large ribosomal subunit protein eL22A Chain: AW
Large ribosomal subunit protein uL14A Chain: AX
Large ribosomal subunit protein eL24A Chain: AY
Large ribosomal subunit protein uL23 Chain: AZ
Large ribosomal subunit protein uL24A Chain: Aa
Large ribosomal subunit protein eL27A Chain: Ab
Large ribosomal subunit protein uL15 Chain: Ac
Large ribosomal subunit protein eL29 Chain: Ad
Large ribosomal subunit protein eL30 Chain: Ae
Large ribosomal subunit protein eL31A Chain: Af
Large ribosomal subunit protein eL32 Chain: Ag
Large ribosomal subunit protein eL33A Chain: Ah
Large ribosomal subunit protein eL34A Chain: Ai
Large ribosomal subunit protein uL29A Chain: Aj
Large ribosomal subunit protein eL36A Chain: Ak
Large ribosomal subunit protein eL37A Chain: Al
Large ribosomal subunit protein eL38 Chain: Am
Large ribosomal subunit protein eL39 Chain: An
Ubiquitin-ribosomal protein eL40A fusion protein Chain: Ao
Small ribosomal subunit protein eS32B Chain: Ap
Large ribosomal subunit protein eL42A Chain: Aq
Large ribosomal subunit protein eL43A Chain: Ar
Small ribosomal subunit protein uS2A Chain: BB
Small ribosomal subunit protein eS1A Chain: BC
Small ribosomal subunit protein uS5 Chain: BD
Small ribosomal subunit protein uS3 Chain: BE
Small ribosomal subunit protein eS4A Chain: BF
Rps5p Chain: BG
Small ribosomal subunit protein eS6A Chain: BH
Small ribosomal subunit protein eS7A Chain: BI
Small ribosomal subunit protein eS8A Chain: BJ
Small ribosomal subunit protein uS4A Chain: BK
Small ribosomal subunit protein eS10A Chain: BL
Small ribosomal subunit protein uS17A Chain: BM
Small ribosomal subunit protein eS12 Chain: BN
Small ribosomal subunit protein uS15 Chain: BO
Small ribosomal subunit protein uS11A Chain: BP
Small ribosomal subunit protein uS19 Chain: BQ
Small ribosomal subunit protein uS9A Chain: BR
Small ribosomal subunit protein eS17A Chain: BS
Small ribosomal subunit protein uS13A Chain: BT
Small ribosomal subunit protein eS19A Chain: BU
Small ribosomal subunit protein uS10 Chain: BV
Small ribosomal subunit protein eS21A Chain: BW
Small ribosomal subunit protein uS8A Chain: BX
Small ribosomal subunit protein uS12A Chain: BY
Small ribosomal subunit protein eS24A Chain: BZ
Small ribosomal subunit protein eS25A Chain: Ba
Small ribosomal subunit protein eS26B Chain: Bb
Small ribosomal subunit protein eS27A Chain: Bc
Small ribosomal subunit protein eS28A Chain: Bd
Small ribosomal subunit protein uS14A Chain: Be
Small ribosomal subunit protein eS30A Chain: Bf
Ubiquitin-ribosomal protein eS31 fusion protein Chain: Bg
Small ribosomal subunit protein RACK1 Chain: Bh
[NU+] prion formation protein 1 Chain: Bi
28S ribosomal RNA Chain: AA
5S ribosomal RNA Chain: AB
5.8S ribosomal RNA Chain: AC
18S rRNA (1707-MER) Chain: BA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.28Å
Relevant EMDB volumes: EMD-10098
Expression system: Escherichia coli