› 6tl4

X-ray diffraction
2.9Å resolution

Photosensory module (PAS-GAF-PHY) of Glycine max phyB

Released:
DOI: 10.2210/pdb6tl4/pdb
PDB entry 6tl4 coloured by chain, front view.
PDB entry 6tl4 coloured by chain, side view.
PDB entry 6tl4 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Structural insights into photoactivation and signalling in plant phytochromes.
Nagano S, Guan K, Shenkutie SM, Feiler C, Weiss M, Kraskov A, Buhrke D, Hildebrandt P, Hughes J
Nat Plants 6 581-588 (2020)
PMID: 32366982

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytochrome B-2 Chain: A
Molecule details ›
Chain: A
Length: 539 amino acids
Theoretical weight: 59.9 KDa
Source organism: Glycine max
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I1MGE5 (Residues: 85-616; Coverage: 46%)
Gene name: GLYMA_15G140000
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CYC 1 x CYC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 102.269Å b: 102.269Å c: 222.918Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.257 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Vibrational Spectroscopy of Phytochromes.
Hildebrandt P. (2023)
12 other articles

3 mentions without citation

The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.
Tang et al. (2021)
2 more