7b93

Electron Microscopy
3.04Å resolution

Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A

Released:
Source organism: Mus musculus
Related structures: EMD-12095

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 45-mer (preferred)
PDBe Complex ID:
PDB-CPX-124487 (preferred)
Entry contents:
44 distinct polypeptide molecules
Macromolecules (44 distinct):
NADH-ubiquinone oxidoreductase chain 3 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.25 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P03899 (Residues: 1-115; Coverage: 100%)
Gene names: Nd3, mt-Nd3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 224 amino acids
Theoretical weight: 24.72 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DC70 (Residues: 1-224; Coverage: 100%)
Gene name: Ndufs7
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: C
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Chain: D
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: E
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: F
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: G
NADH-ubiquinone oxidoreductase chain 1 Chain: H
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Chain: I
NADH-ubiquinone oxidoreductase chain 6 Chain: J
NADH-ubiquinone oxidoreductase chain 4L Chain: K
NADH-ubiquinone oxidoreductase chain 5 Chain: L
NADH-ubiquinone oxidoreductase chain 4 Chain: M
NADH-ubiquinone oxidoreductase chain 2 Chain: N
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: O
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Chain: P
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: Q
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: R
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: S
Acyl carrier protein, mitochondrial Chains: T, U
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Chain: V
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: W
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: X
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Chain: Y
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: Z
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: a
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Chain: b
NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Chain: c
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: d
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Chain: e
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Chain: f
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial Chain: g
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Chain: h
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: i
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Chain: j
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Chain: k
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: l
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: m
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Chain: n
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Chain: o
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Chain: p
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: q
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: r
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Chain: s

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-12095