7egs

X-ray diffraction
1.7Å resolution

The crystal structure of lobe domain of E. coli RNA polymerase complexed with the C-terminal domain of UvrD

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-136463 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 152-443; Coverage: 22%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA helicase II Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03018 (Residues: 654-720; Coverage: 9%)
Gene names: JW3786, b3813, mutU, pdeB, rad, recL, uvrD
Sequence domains: PcrA/UvrD tudor domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 60.085Å b: 42.241Å c: 87.849Å
α: 90° β: 105.23° γ: 90°
R-values:
R R work R free
0.205 0.203 0.234
Expression system: Escherichia coli BL21(DE3)