› 7l1a

X-ray diffraction
1.25Å resolution

Human Methionine Adenosyltransferase 2A bound to Methylthioadenosine and inhibitor, di-imido triphosphate (PNPNP)

Released:
DOI: 10.2210/pdb7l1a/pdb
PDB entry 7l1a coloured by chain, front view.
PDB entry 7l1a coloured by chain, side view.
PDB entry 7l1a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism and Inhibition of Human Methionine Adenosyltransferase 2A.
Niland CN, Ghosh A, Cahill SM, Schramm VL
Biochemistry 60 791-801 (2021)
PMID: 33656855

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-2 Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31153 (Residues: 1-395; Coverage: 100%)
Gene names: AMS2, MAT2A, MATA2
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand MG 2 x MG
Ligand K 1 x K
Ligand XE1 1 x XE1
Ligand MTA 1 x MTA
Ligand ALA 1 x ALA
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 68.113Å b: 94.148Å c: 117.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.141
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Analogs of S-Adenosyl-L-Methionine in Studies of Methyltransferases.
Rudenko et al. (2022)
2 more

3 mentions without citation

Mechanism and Inhibition of Human Methionine Adenosyltransferase 2A.
Niland et al. (2021)
2 more