7n8b

Electron Microscopy
3.05Å resolution

Cycloheximide bound vacant 80S structure isolated from cbf5-D95A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 79-mer (preferred)
PDBe Complex ID:
PDB-CPX-126797 (preferred)
Entry contents:
75 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (79 distinct):
Large ribosomal subunit protein uL2A Chain: AA
Molecule details ›
Chain: AA
Length: 247 amino acids
Theoretical weight: 26.64 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX45 (Residues: 2-248; Coverage: 97%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains:
Large ribosomal subunit protein uL3 Chain: AB
Molecule details ›
Chain: AB
Length: 386 amino acids
Theoretical weight: 43.73 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14126 (Residues: 2-387; Coverage: 100%)
Gene names: MAK8, RPL3, TCM1, YOR063W, YOR29-14
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4A Chain: AC
Large ribosomal subunit protein uL18 Chain: AD
Large ribosomal subunit protein eL6A Chain: AE
Large ribosomal subunit protein uL30A Chain: AF
Large ribosomal subunit protein eL8A Chain: AG
Large ribosomal subunit protein uL6A Chain: AH
Large ribosomal subunit protein uL16 Chain: AI
Large ribosomal subunit protein uL5A Chain: AJ
Large ribosomal subunit protein eL13A Chain: AL
Large ribosomal subunit protein eL14A Chain: AM
Large ribosomal subunit protein eL15A Chain: AN
Large ribosomal subunit protein uL13A Chain: AO
Large ribosomal subunit protein uL22A Chain: AP
Large ribosomal subunit protein eL18A Chain: AQ
Large ribosomal subunit protein eL19A Chain: AR
Large ribosomal subunit protein eL20A Chain: AS
Large ribosomal subunit protein eL21A Chain: AT
Large ribosomal subunit protein eL22A Chain: AU
Large ribosomal subunit protein uL14A Chain: AV
Large ribosomal subunit protein eL24A Chain: AW
Large ribosomal subunit protein uL23 Chain: AX
Large ribosomal subunit protein uL24A Chain: AY
Large ribosomal subunit protein eL27A Chain: AZ
Large ribosomal subunit protein uL15 Chain: Aa
Large ribosomal subunit protein eL29 Chain: Ab
Large ribosomal subunit protein eL30 Chain: Ac
Large ribosomal subunit protein eL31A Chain: Ad
Large ribosomal subunit protein eL32 Chain: Ae
Large ribosomal subunit protein eL33A Chain: Af
Large ribosomal subunit protein eL34A Chain: Ag
Large ribosomal subunit protein uL29A Chain: Ah
Large ribosomal subunit protein eL36A Chain: Ai
Large ribosomal subunit protein eL37A Chain: Aj
Large ribosomal subunit protein eL38 Chain: Ak
Large ribosomal subunit protein eL39 Chain: Al
Large ribosomal subunit protein eL40A Chain: Am
Small ribosomal subunit protein eS32A Chain: An
Large ribosomal subunit protein eL42A Chain: Ao
Large ribosomal subunit protein eL43A Chain: Ap
Small ribosomal subunit protein uS2A Chain: BA
Small ribosomal subunit protein eS1A Chain: BB
Small ribosomal subunit protein uS5 Chain: BC
Small ribosomal subunit protein uS3 Chain: BD
Small ribosomal subunit protein eS4A Chain: BE
Small ribosomal subunit protein uS7 Chain: BF
Small ribosomal subunit protein eS6A Chain: BG
Small ribosomal subunit protein eS7A Chain: BH
Small ribosomal subunit protein eS8A Chain: BI
Small ribosomal subunit protein uS4A Chain: BJ
Small ribosomal subunit protein eS10A Chain: BK
Small ribosomal subunit protein uS17A Chain: BL
Small ribosomal subunit protein eS12 Chain: BM
Small ribosomal subunit protein uS15 Chain: BN
Small ribosomal subunit protein uS11A Chain: BO
Small ribosomal subunit protein uS19 Chain: BP
Small ribosomal subunit protein uS9A Chain: BQ
Small ribosomal subunit protein eS17A Chain: BR
Small ribosomal subunit protein uS13A Chain: BS
Small ribosomal subunit protein eS19A Chain: BT
Small ribosomal subunit protein uS10 Chain: BU
Small ribosomal subunit protein eS21A Chain: BV
Small ribosomal subunit protein uS8A Chain: BW
Small ribosomal subunit protein uS12A Chain: BX
Small ribosomal subunit protein eS24A Chain: BY
Small ribosomal subunit protein eS25A Chain: BZ
Small ribosomal subunit protein eS26B Chain: Ba
Small ribosomal subunit protein eS27A Chain: Bb
Small ribosomal subunit protein eS28A Chain: Bc
Small ribosomal subunit protein uS14A Chain: Bd
Small ribosomal subunit protein eS30A Chain: Be
Small ribosomal subunit protein eS31 Chain: Bf
Small ribosomal subunit protein RACK1 Chain: Bg
Suppressor protein STM1 Chain: Bh
25S Chain: A1
5S Chain: A3
5.8S Chain: A4
18S Ribosomal RNA Chain: B5

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-24235