7nrd

Electron Microscopy
4.36Å resolution

Structure of the yeast Gcn1 bound to a colliding stalled 80S ribosome with MBF1, A/P-tRNA and P/E-tRNA

Released:
Related structures: EMD-12535

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 82-mer (preferred)
PDBe Complex ID:
PDB-CPX-249653 (preferred)
Entry contents:
75 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (82 distinct):
Small ribosomal subunit protein uS3 Chain: SA
Molecule details ›
Chain: SA
Length: 223 amino acids
Theoretical weight: 24.7 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05750 (Residues: 3-225; Coverage: 93%)
Gene names: N1653, RPS3, SUF14, YNL178W
Sequence domains:
Small ribosomal subunit protein uS7 Chain: SB
Molecule details ›
Chain: SB
Length: 206 amino acids
Theoretical weight: 22.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P26783 (Residues: 20-225; Coverage: 92%)
Gene names: J2045, RPS2, RPS5, YJR123W
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein eS10A Chain: SC
Small ribosomal subunit protein eS12 Chain: SD
Small ribosomal subunit protein uS19 Chain: SE
Small ribosomal subunit protein uS9A Chain: SF
Small ribosomal subunit protein eS17B Chain: SG
Small ribosomal subunit protein uS13A Chain: SH
Small ribosomal subunit protein eS19A Chain: SI
Small ribosomal subunit protein uS10 Chain: SJ
Small ribosomal subunit protein eS25A Chain: SK
Small ribosomal subunit protein eS28A Chain: SL
Small ribosomal subunit protein uS14A Chain: SM
Small ribosomal subunit protein eS31 Chain: SN
Small ribosomal subunit protein RACK1 Chain: SO
Small ribosomal subunit protein uS2A Chain: SP
Small ribosomal subunit protein eS1A Chain: SQ
Small ribosomal subunit protein uS5 Chain: SR
Small ribosomal subunit protein eS4A Chain: SS
Small ribosomal subunit protein eS6A Chain: ST
Small ribosomal subunit protein eS7A Chain: SU
Small ribosomal subunit protein eS8A Chain: SV
Small ribosomal subunit protein uS4A Chain: SW
Small ribosomal subunit protein uS17A Chain: SX
Small ribosomal subunit protein uS15 Chain: SY
Small ribosomal subunit protein uS11B Chain: SZ
Small ribosomal subunit protein eS21A Chain: Sa
Small ribosomal subunit protein uS8A Chain: Sb
Small ribosomal subunit protein uS12A Chain: Sc
Small ribosomal subunit protein eS24A Chain: Sd
Small ribosomal subunit protein eS26B Chain: Se
Small ribosomal subunit protein eS27A Chain: Sf
Small ribosomal subunit protein eS30A Chain: Sg
Multiprotein-bridging factor 1 Chain: Sh
Large ribosomal subunit protein uL2A Chain: LD
Large ribosomal subunit protein uL3 Chain: LE
Large ribosomal subunit protein uL4A Chain: LF
Large ribosomal subunit protein uL18 Chain: LG
Large ribosomal subunit protein eL6B Chain: LH
Large ribosomal subunit protein uL30A Chain: LI
Large ribosomal subunit protein eL8A Chain: LJ
Large ribosomal subunit protein uL6A Chain: LK
Large ribosomal subunit protein uL16 Chain: LL
Large ribosomal subunit protein uL5B Chain: LM
Large ribosomal subunit protein eL13A Chain: LN
Large ribosomal subunit protein eL14A Chain: LO
Large ribosomal subunit protein eL15A Chain: LP
Large ribosomal subunit protein uL13A Chain: LQ
Large ribosomal subunit protein uL22A Chain: LR
Large ribosomal subunit protein eL18A Chain: LS
Large ribosomal subunit protein eL19A Chain: LT
Large ribosomal subunit protein eL20A Chain: LU
Large ribosomal subunit protein eL21A Chain: LV
Large ribosomal subunit protein eL22A Chain: LW
Large ribosomal subunit protein uL14A Chain: LX
Large ribosomal subunit protein eL24A Chain: LY
Large ribosomal subunit protein uL23 Chain: LZ
Large ribosomal subunit protein uL24A Chain: La
Large ribosomal subunit protein eL27A Chain: Lb
Large ribosomal subunit protein uL15 Chain: Lc
Large ribosomal subunit protein eL29 Chain: Ld
Large ribosomal subunit protein eL30 Chain: Le
Large ribosomal subunit protein eL31A Chain: Lf
Large ribosomal subunit protein eL32 Chain: Lg
Large ribosomal subunit protein eL33A Chain: Lh
Large ribosomal subunit protein eL34A Chain: Li
Large ribosomal subunit protein uL29A Chain: Lj
Large ribosomal subunit protein eL36A Chain: Lk
Large ribosomal subunit protein eL37A Chain: Ll
Large ribosomal subunit protein eL38 Chain: Lm
Large ribosomal subunit protein eL39 Chain: Ln
Large ribosomal subunit protein eL40A Chain: Lo
Small ribosomal subunit protein eS32B Chain: Lp
Large ribosomal subunit protein eL42A Chain: Lq
Large ribosomal subunit protein eL43A Chain: Lr
TPA_inf: Saccharomyces cerevisiae S288C chromosome XII, complete sequence Chain: S2
mRNA (32-MER) Chain: S3
tRNA (75-MER) Chain: Sn
tRNA (77-MER) Chain: Sm
25S rRNA (3184-MER) Chain: LA
5S rRNA (121-MER) Chain: LB
5.8S rRNA (158-MER) Chain: LC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.36Å
Relevant EMDB volumes: EMD-12535