7oj0

Electron Microscopy
3.5Å resolution

Cryo-EM structure of 70S ribosome stalled with TnaC peptide and RF2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 59-mer (preferred)
PDBe Complex ID:
PDB-CPX-279454 (preferred)
Entry contents:
54 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (59 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bS21 Chain: U
Large ribosomal subunit protein uL2 Chain: c
Large ribosomal subunit protein uL3 Chain: d
Large ribosomal subunit protein uL4 Chain: e
Large ribosomal subunit protein uL5 Chain: f
Large ribosomal subunit protein uL6 Chain: g
Large ribosomal subunit protein bL9 Chain: h
Large ribosomal subunit protein uL13 Chain: i
Large ribosomal subunit protein uL14 Chain: j
Large ribosomal subunit protein uL15 Chain: k
Large ribosomal subunit protein uL16 Chain: l
Large ribosomal subunit protein bL17 Chain: m
Large ribosomal subunit protein uL18 Chain: n
Large ribosomal subunit protein bL19 Chain: o
Large ribosomal subunit protein bL20 Chain: p
Large ribosomal subunit protein bL21 Chain: q
Large ribosomal subunit protein uL22 Chain: r
Large ribosomal subunit protein uL23 Chain: s
Large ribosomal subunit protein uL24 Chain: t
Large ribosomal subunit protein bL25 Chain: u
Large ribosomal subunit protein bL27 Chain: v
Large ribosomal subunit protein bL28 Chain: w
Large ribosomal subunit protein uL29 Chain: x
Large ribosomal subunit protein uL30 Chain: y
Large ribosomal subunit protein bL32 Chain: z
Large ribosomal subunit protein bL33 Chain: 0
Large ribosomal subunit protein bL34 Chain: 1
Large ribosomal subunit protein bL35 Chain: 2
Large ribosomal subunit protein bL36A Chain: 3
Large ribosomal subunit protein bL31 Chain: 4
Tryptophanase operon leader peptide Chain: 7
Alternative ribosome-rescue factor A Chain: 6
Peptide chain release factor RF2 Chain: 8
Large ribosomal subunit protein uL10 Chain: 5
Large ribosomal subunit protein uL11 Chain: 9
16S ribosomal RNA Chain: A
23S ribosomal RNA Chain: a
5S ribosomal RNA Chain: b
Messenger RNA Chain: X
tRNA Chain: V

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-12937