7q4p

Electron Microscopy
2.15Å resolution

U2 snRNP after ATP-dependent remodelling

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis of branch site recognition by the human spliceosome.
Science 375 50-57 (2022)
PMID: 34822310
Related structures: EMD-13812

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-131043 (preferred)
Entry contents:
7 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Splicing factor 3A subunit 2 Chain: 1
Molecule details ›
Chain: 1
Length: 464 amino acids
Theoretical weight: 49.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15428 (Residues: 1-464; Coverage: 100%)
Gene names: SAP62, SF3A2
Sequence domains:
Splicing factor 3A subunit 3 Chain: 9
Molecule details ›
Chain: 9
Length: 501 amino acids
Theoretical weight: 58.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q12874 (Residues: 1-501; Coverage: 100%)
Gene names: SAP61, SF3A3
Sequence domains:
Splicing factor 3B subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1304 amino acids
Theoretical weight: 146.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75533 (Residues: 1-1304; Coverage: 100%)
Gene names: SAP155, SF3B1
Sequence domains:
Splicing factor 3B subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 895 amino acids
Theoretical weight: 100.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13435 (Residues: 1-895; Coverage: 100%)
Gene names: SAP145, SF3B2
Sequence domains:
Splicing factor 3B subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 1217 amino acids
Theoretical weight: 135.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15393 (Residues: 1-1217; Coverage: 100%)
Gene names: KIAA0017, SAP130, SF3B3
Sequence domains:
Splicing factor 3B subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 86 amino acids
Theoretical weight: 10.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BWJ5 (Residues: 1-86; Coverage: 100%)
Gene names: SF3B10, SF3B5
Sequence domains: Splicing factor 3B subunit 10 (SF3b10)
PHD finger-like domain-containing protein 5A Chain: G
Molecule details ›
Chain: G
Length: 110 amino acids
Theoretical weight: 12.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q7RTV0 (Residues: 1-110; Coverage: 100%)
Gene name: PHF5A
Sequence domains: PHF5-like protein
U2 snRNA Chain: 2
Molecule details ›
Chain: 2
Length: 188 nucleotides
Theoretical weight: 60.27 KDa
Sequence domains: U2 spliceosomal RNA

Ligands and Environments

1 bound ligand:
4 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 2.15Å
Relevant EMDB volumes: EMD-13812