7qo3

Electron Microscopy
6.1Å resolution

Structure of the 26S proteasome-Ubp6 complex in the si state (Core Particle and Lid)

Released:

Function and Biology Details

Reactions catalysed:
Cleavage of peptide bonds with very broad specificity.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 41-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126893 (preferred)
Entry contents:
27 distinct polypeptide molecules
Macromolecules (27 distinct):
Proteasome subunit alpha type-1 Chains: A, a
Molecule details ›
Chains: A, a
Length: 252 amino acids
Theoretical weight: 28.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21243 (Residues: 1-252; Coverage: 100%)
Gene names: PRC2, PRS2, SCL1, YGL011C
Sequence domains:
Proteasome subunit alpha type-2 Chains: B, b
Molecule details ›
Chains: B, b
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23639 (Residues: 1-250; Coverage: 100%)
Gene names: PRE8, PRS4, YML092C
Sequence domains:
Proteasome subunit alpha type-3 Chains: C, c
Proteasome subunit alpha type-4 Chains: D, d
Proteasome subunit alpha type-5 Chains: E, e
Proteasome subunit alpha type-6 Chains: F, f
Probable proteasome subunit alpha type-7 Chains: G, g
Proteasome subunit beta type-1 Chains: 1, h
Proteasome subunit beta type-2 Chains: 2, i
Proteasome subunit beta type-3 Chains: 3, j
Proteasome subunit beta type-4 Chains: 4, k
Proteasome subunit beta type-5 Chains: 5, l
Proteasome subunit beta type-6 Chains: 6, m
Proteasome subunit beta type-7 Chains: 7, n
26S proteasome regulatory subunit RPN10 Chain: W
Ubiquitin carboxyl-terminal hydrolase RPN11 Chain: V
26S proteasome regulatory subunit RPN12 Chain: T
26S proteasome regulatory subunit RPN13 Chain: X
26S proteasome complex subunit SEM1 Chain: Y
26S proteasome regulatory subunit RPN1 Chain: Z
26S proteasome regulatory subunit RPN2 Chain: N
26S proteasome regulatory subunit RPN3 Chain: S
26S proteasome regulatory subunit RPN5 Chain: P
26S proteasome regulatory subunit RPN6 Chain: Q
26S proteasome regulatory subunit RPN7 Chain: R
26S proteasome regulatory subunit RPN8 Chain: U
26S proteasome regulatory subunit RPN9 Chain: O

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.1Å
Relevant EMDB volumes: EMD-14082