7u5c

Electron Microscopy
4.6Å resolution

Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state

Released:
Source organism: Homo sapiens
Related structures: EMD-26346

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-122144 (preferred)
Entry contents:
7 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (8 distinct):
DNA primase small subunit Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49642 (Residues: 1-420; Coverage: 100%)
Gene name: PRIM1
Sequence domains: DNA primase small subunit
DNA primase large subunit Chain: B
Molecule details ›
Chain: B
Length: 512 amino acids
Theoretical weight: 59.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
DNA polymerase alpha catalytic subunit Chain: C
DNA polymerase alpha subunit B Chain: D
CST complex subunit CTC1 Chain: E
CST complex subunit STN1 Chain: F
CST complex subunit TEN1 Chain: G
canonical telomeric DNA sequence Chain: H

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-26346
Expression system: Trichoplusia ni