7uio

Electron Microscopy
3.3Å resolution

Mediator-PIC Early (Composite Model)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 80-mer (preferred)
PDBe Complex ID:
PDB-CPX-512166 (preferred)
Entry contents:
39 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (41 distinct):
Mediator of RNA polymerase II transcription subunit 15 Chains: Ao, Bo
Molecule details ›
Chains: Ao, Bo
Length: 1081 amino acids
Theoretical weight: 120.43 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P19659 (Residues: 1-1081; Coverage: 100%)
Gene names: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W
Sequence domains:
Mediator of RNA polymerase II transcription subunit 2 Chains: Ab, Bb
Molecule details ›
Chains: Ab, Bb
Length: 431 amino acids
Theoretical weight: 47.75 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12124 (Residues: 1-431; Coverage: 100%)
Gene names: D2930, MED2, YDL005C
Sequence domains: Mediator complex subunit 2
Mediator of RNA polymerase II transcription subunit 3 Chains: Ac, Bc
Mediator of RNA polymerase II transcription subunit 14 Chains: An, Bn
Mediator of RNA polymerase II transcription subunit 5 Chains: Ae, Be
Mediator of RNA polymerase II transcription subunit 16 Chains: Ap, Bp
Regulatory protein GAL4 Chains: GA, GB
Mediator of RNA polymerase II transcription subunit 20 Chains: At, Bt
Mediator of RNA polymerase II transcription subunit 7 Chains: Ag, Bg
Mediator of RNA polymerase II transcription subunit 8 Chains: Ah, Bh
Mediator of RNA polymerase II transcription subunit 9 Chains: Ai, Bi
Mediator of RNA polymerase II transcription subunit 10 Chains: Aj, Bj
Mediator of RNA polymerase II transcription subunit 11 Chains: Ak, Bk
Mediator of RNA polymerase II transcription subunit 17 Chains: Aq, Bq
Mediator of RNA polymerase II transcription subunit 18 Chains: Ar, Br
Mediator of RNA polymerase II transcription subunit 19 Chains: As, Bs
Mediator of RNA polymerase II transcription subunit 21 Chains: Au, Bu
Mediator of RNA polymerase II transcription subunit 22 Chains: Av, Bv
Mediator of RNA polymerase II transcription subunit 31 Chains: Aw, Bw
DNA-directed RNA polymerase II subunit RPB1 Chains: Az, Bz
DNA-directed RNA polymerase II subunit RPB1 Chains: AA, BA
DNA-directed RNA polymerase II subunit RPB2 Chains: AB, BB
DNA-directed RNA polymerase II subunit RPB3 Chains: AC, BC
DNA-directed RNA polymerase II subunit RPB4 Chains: AD, BD
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chains: AE, BE
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chains: AF, BF
DNA-directed RNA polymerase II subunit RPB7 Chains: AG, BG
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chains: AH, BH
DNA-directed RNA polymerase II subunit RPB9 Chains: AI, BI
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chains: AJ, BJ
DNA-directed RNA polymerase II subunit RPB11 Chains: AK, BK
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chains: AL, BL
Transcription initiation factor IIB Chains: AM, BM
Transcription initiation factor IIF subunit alpha Chains: AP, BP
Transcription initiation factor IIF subunit beta Chains: AQ, BQ
Transcription elongation factor S-II Chains: AS, BS
Mediator of RNA polymerase II transcription subunit 1 Chains: Aa, Ba
Mediator of RNA polymerase II transcription subunit 4 Chains: Ad, Bd
Mediator of RNA polymerase II transcription subunit 6 Chains: Af, Bf
DNA (38-MER) Chain: A
DNA (37-MER) Chain: B

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-26551
Expression system: Escherichia coli