7uph

Electron Microscopy
4.18Å resolution

Structure of a ribosome with tethered subunits

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 49-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-249897 (preferred)
Entry contents:
47 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (49 distinct):
Small ribosomal subunit protein uS14 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 2-89; Coverage: 99%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: C
Small ribosomal subunit protein uS17 Chain: D
Small ribosomal subunit protein bS18 Chain: E
Small ribosomal subunit protein uS19 Chain: F
Small ribosomal subunit protein bS20 Chain: G
Small ribosomal subunit protein bS21 Chain: H
Large ribosomal subunit protein uL2 Chain: K
Large ribosomal subunit protein uL3 Chain: L
Large ribosomal subunit protein uL4 Chain: M
Large ribosomal subunit protein uL5 Chain: N
Large ribosomal subunit protein uL6 Chain: O
Large ribosomal subunit protein uL13 Chain: R
Large ribosomal subunit protein uL14 Chain: S
Large ribosomal subunit protein uL15 Chain: T
Large ribosomal subunit protein uL16 Chain: U
Large ribosomal subunit protein bL17 Chain: V
Small ribosomal subunit protein uS2 Chain: W
Small ribosomal subunit protein uS3 Chain: X
Small ribosomal subunit protein uS4 Chain: Y
Small ribosomal subunit protein uS5 Chain: Z
Small ribosomal subunit protein bS6, non-modified isoform Chain: a
Small ribosomal subunit protein uS7 Chain: b
Small ribosomal subunit protein uS8 Chain: c
Small ribosomal subunit protein uS9 Chain: d
Small ribosomal subunit protein uS10 Chain: e
Small ribosomal subunit protein uS11 Chain: f
Small ribosomal subunit protein uS12 Chain: g
Small ribosomal subunit protein uS13 Chain: h
Large ribosomal subunit protein bL32 Chain: i
Large ribosomal subunit protein bL33 Chain: j
Large ribosomal subunit protein bL34 Chain: k
Large ribosomal subunit protein bL35 Chain: l
Large ribosomal subunit protein bL36A Chain: m
Large ribosomal subunit protein uL18 Chain: n
Large ribosomal subunit protein bL19 Chain: o
Large ribosomal subunit protein bL20 Chain: p
Large ribosomal subunit protein bL21 Chain: q
Large ribosomal subunit protein uL22 Chain: r
Large ribosomal subunit protein uL23 Chain: s
Large ribosomal subunit protein uL24 Chain: t
Large ribosomal subunit protein bL25 Chain: u
Large ribosomal subunit protein bL27 Chain: v
Large ribosomal subunit protein bL28 Chain: w
Large ribosomal subunit protein uL29 Chain: x
Large ribosomal subunit protein uL30 Chain: y
Tethered rRNA Chain: I
5S ribosomal RNA Chain: J

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 4.18Å
Relevant EMDB volumes: EMD-26666
Expression system: Escherichia coli