7w3i

Electron Microscopy
3.5Å resolution

Structure of USP14-bound human 26S proteasome in substrate-inhibited state SB_USP14

Released:
Source organism: Homo sapiens
Related structures: EMD-32281

Function and Biology Details

Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100677 (preferred)
Entry contents:
34 distinct polypeptide molecules
Macromolecules (34 distinct):
Ubiquitin carboxyl-terminal hydrolase 14 Chain: x
Molecule details ›
Chain: x
Length: 494 amino acids
Theoretical weight: 56.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54578 (Residues: 1-494; Coverage: 100%)
Gene names: TGT, USP14
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 1-433; Coverage: 100%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: B
26S proteasome regulatory subunit 8 Chain: C
26S proteasome regulatory subunit 6B Chain: D
AAA+ ATPase domain-containing protein Chain: E
26S proteasome regulatory subunit 6A Chain: F
Proteasome subunit alpha type-6 Chains: G, g
Proteasome subunit alpha type-2 Chains: H, h
Proteasome subunit alpha type-4 Chains: I, i
Proteasome subunit alpha type-7 Chains: J, j
Proteasome subunit alpha type-5 Chains: K, k
Proteasome subunit alpha type-1 Chains: L, l
Proteasome subunit alpha type-3 Chains: M, m
Proteasome subunit beta type-6 Chains: N, n
Proteasome subunit beta type-7 Chains: O, o
Proteasome subunit beta type-3 Chains: P, p
Proteasome subunit beta type-2 Chains: Q, q
Proteasome subunit beta type-5 Chains: R, r
Proteasome subunit beta type-1 Chains: S, s
Proteasome subunit beta type-4 Chains: T, t
Ubiquitin Chain: y
26S proteasome non-ATPase regulatory subunit 1 Chain: U
26S proteasome non-ATPase regulatory subunit 3 Chain: V
26S proteasome non-ATPase regulatory subunit 12 Chain: W
26S proteasome non-ATPase regulatory subunit 11 Chain: X
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
26S proteasome non-ATPase regulatory subunit 13 Chain: a
26S proteasome non-ATPase regulatory subunit 4 Chain: b
26S proteasome non-ATPase regulatory subunit 14 Chain: c
26S proteasome non-ATPase regulatory subunit 8 Chain: d
26S proteasome non-ATPase regulatory subunit 2 Chain: f
26S proteasome complex subunit SEM1 Chain: e

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-32281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens