8bh4

Electron Microscopy
2.62Å resolution

Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAm6A mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site

Released:
Source organism: Escherichia coli K-12
Related structures: EMD-16047

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-272107 (preferred)
Entry contents:
49 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: C
Large ribosomal subunit protein uL5 Chain: D
Large ribosomal subunit protein uL6 Chain: E
Large ribosomal subunit protein bL9 Chain: F
Large ribosomal subunit protein uL13 Chain: G
Large ribosomal subunit protein uL14 Chain: H
Large ribosomal subunit protein uL15 Chain: I
Large ribosomal subunit protein uL16 Chain: J
Large ribosomal subunit protein bL17 Chain: K
Large ribosomal subunit protein uL18 Chain: L
Large ribosomal subunit protein bL19 Chain: M
Large ribosomal subunit protein bL20 Chain: N
Large ribosomal subunit protein bL21 Chain: O
Large ribosomal subunit protein uL22 Chain: P
Large ribosomal subunit protein uL23 Chain: Q
Large ribosomal subunit protein uL24 Chain: R
Large ribosomal subunit protein bL25 Chain: S
Large ribosomal subunit protein bL27 Chain: T
Large ribosomal subunit protein bL28 Chain: U
Large ribosomal subunit protein uL29 Chain: V
Large ribosomal subunit protein uL30 Chain: W
Large ribosomal subunit protein bL32 Chain: a
Large ribosomal subunit protein bL33 Chain: b
Large ribosomal subunit protein bL34 Chain: c
Large ribosomal subunit protein bL35 Chain: d
Large ribosomal subunit protein bL36A Chain: e
Small ribosomal subunit protein uS2 Chain: f
Small ribosomal subunit protein uS3 Chain: g
Small ribosomal subunit protein uS4 Chain: h
Small ribosomal subunit protein uS5 Chain: i
Small ribosomal subunit protein bS6 Chain: j
Small ribosomal subunit protein uS7 Chain: k
Small ribosomal subunit protein uS8 Chain: l
Small ribosomal subunit protein uS9 Chain: m
Small ribosomal subunit protein uS10 Chain: n
Small ribosomal subunit protein uS11 Chain: o
Small ribosomal subunit protein uS12 Chain: p
Small ribosomal subunit protein uS13 Chain: q
Small ribosomal subunit protein uS14 Chain: r
Small ribosomal subunit protein uS15 Chain: s
Small ribosomal subunit protein bS16 Chain: t
Small ribosomal subunit protein uS17 Chain: u
Small ribosomal subunit protein bS18 Chain: v
Small ribosomal subunit protein uS19 Chain: w
Small ribosomal subunit protein bS20 Chain: x
Small ribosomal subunit protein bS21 Chain: y
fMet-Lys dipeptide Chain: 6
23S rRNA RRLG-RRNA Chain: 0
5S ribosomal RNA Chain: 1
16S rRNA RRSB-RRNA Chain: 2
Messenger RNA Chain: 3
Deacylated P-site tRNA(fMet) Chain: 4
Dipeptidyl A-site tRNA(Lys) Chain: 5

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.62Å
Relevant EMDB volumes: EMD-16047