8c83

Electron Microscopy
3Å resolution

Cryo-EM structure of in vitro reconstituted Otu2-bound Ub-40S complex

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-271539 (preferred)
Entry contents:
17 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (18 distinct):
OTU domain-containing protein 2 Chain: x
Molecule details ›
Chain: x
Length: 307 amino acids
Theoretical weight: 36.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38747 (Residues: 1-307; Coverage: 100%)
Gene names: OTU2, YHL013C
Sequence domains: OTU-like cysteine protease
Ubiquitin Chain: y
Molecule details ›
Chain: y
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P05759 (Residues: 1-76; Coverage: 50%)
Gene names: L9470.14, RPS31, RPS37, UBI3, YLR167W
Sequence domains: Ubiquitin family
Small ribosomal subunit protein uS2A Chain: P
Small ribosomal subunit protein uS5 Chain: R
Small ribosomal subunit protein eS4A Chain: S
Small ribosomal subunit protein eS6A Chain: T
Small ribosomal subunit protein eS7A Chain: U
Small ribosomal subunit protein eS8B Chain: V
Small ribosomal subunit protein uS4A Chain: W
Small ribosomal subunit protein uS17A Chain: X
Small ribosomal subunit protein uS15 Chain: Y
Small ribosomal subunit protein eS21A Chain: a
Small ribosomal subunit protein uS8A Chain: b
Small ribosomal subunit protein uS12A Chain: c
Small ribosomal subunit protein eS24A Chain: d
Small ribosomal subunit protein eS27A Chain: f
Small ribosomal subunit protein eS30A Chain: g
18S Ribosomal RNA Chain: 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-16470
Expression system: Escherichia coli