8c8h

Electron Microscopy
3.84Å resolution

Cryo EM structure of the vaccinia complete RNA polymerase complex lacking the capping enzyme

Released:
Source organism: Vaccinia virus GLV-1h68
Entry authors: Grimm G, Bartuli J, Fischer U
Related structures: EMD-16476

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-272261 (preferred)
Entry contents:
12 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (13 distinct):
DNA-directed RNA polymerase 133 kDa polypeptide Chain: B
Molecule details ›
Chain: B
Length: 1164 amino acids
Theoretical weight: 133.53 KDa
Source organism: Vaccinia virus GLV-1h68
UniProt:
  • Canonical: Q76ZP7 (Residues: 1-1164; Coverage: 100%)
Gene names: A24R, OPG151, RPO132, VACWR144
Sequence domains:
DNA-directed RNA polymerase 22 kDa subunit Chain: E
Molecule details ›
Chain: E
Length: 185 amino acids
Theoretical weight: 21.37 KDa
Source organism: Vaccinia virus GLV-1h68
UniProt:
  • Canonical: P68609 (Residues: 1-185; Coverage: 100%)
Gene names: J4R, OPG103, RPO22, VACWR096
Sequence domains: Poxvirus RNA polymerase 22 kDa subunit
DNA-directed RNA polymerase 19 kDa subunit Chain: F
DNA-directed RNA polymerase 18 kDa subunit Chain: G
RNA polymerase-associated transcription-specificity factor RAP94 Chain: I
DNA-directed RNA polymerase 7 kDa subunit Chain: J
Core protein OPG073 Chains: Q, R
Early transcription factor 82 kDa subunit Chain: K
DNA-directed RNA polymerase 147 kDa polypeptide Chain: A
Nucleoside triphosphatase I Chain: Y
DNA-directed RNA polymerase 35 kDa subunit Chain: C
DNA-directed RNA polymerase 30 kDa polypeptide Chain: S
chr17.trna16-GlnTTG Chain: U

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.84Å
Relevant EMDB volumes: EMD-16476