8e49

Electron Microscopy
2.05Å resolution

E. coli 50S ribosome bound to antibiotic analog SLC31

Released:
Source organism: Escherichia coli
Entry authors: Pellegrino J, Lee DJ, Fraser JS, Seiple IB
Related structures: EMD-27884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-232417 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (10 distinct):
Large ribosomal subunit protein uL2 Chain: K
Molecule details ›
Chain: K
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL4 Chain: M
Large ribosomal subunit protein uL3 Chain: N
Large ribosomal subunit protein uL13 Chain: O
Large ribosomal subunit protein uL22 Chain: P
Large ribosomal subunit protein bL32 Chain: Q
Large ribosomal subunit protein bL34 Chain: R
23S ribosomal RNA Chain: I
5S ribosomal RNA Chain: J

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.05Å
Relevant EMDB volumes: EMD-27884
Expression system: Escherichia coli