8evp

Electron Microscopy
2.38Å resolution

Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), Structure I

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Regulation of translation by ribosomal RNA pseudouridylation.
OpenAccess logo Sci Adv 9 eadg8190 (2023)
PMID: 37595043
Related structures: EMD-28632

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 80-mer (preferred)
PDBe Complex ID:
PDB-CPX-268933 (preferred)
Entry contents:
75 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (80 distinct):
Small ribosomal subunit protein uS2A Chain: BA
Molecule details ›
Chain: BA
Length: 252 amino acids
Theoretical weight: 28.05 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32905 (Residues: 1-252; Coverage: 100%)
Gene names: NAB1, NAB1A, RPS0A, YGR214W, YST1
Sequence domains: Ribosomal protein S2
RPS1A isoform 1 Chain: BB
Molecule details ›
Chain: BB
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae
RPS2 isoform 1 Chain: BC
Small ribosomal subunit protein eS4A Chain: BE
Small ribosomal subunit protein eS6A Chain: BG
Small ribosomal subunit protein eS7A Chain: BH
Small ribosomal subunit protein eS8A Chain: BI
Small ribosomal subunit protein uS4A Chain: BJ
Small ribosomal subunit protein uS17A Chain: BL
Small ribosomal subunit protein uS15 Chain: BN
Small ribosomal subunit protein uS11A Chain: BO
Small ribosomal subunit protein eS21A Chain: BV
RPS22A isoform 1 Chain: BW
Small ribosomal subunit protein uS12A Chain: BX
Small ribosomal subunit protein eS24A Chain: BY
RPS26B isoform 1 Chain: Ba
Small ribosomal subunit protein eS27A Chain: Bb
Small ribosomal subunit protein eS30A Chain: Be
RPS3 isoform 1 Chain: BD
Rps5p Chain: BF
Small ribosomal subunit protein eS10A Chain: BK
RPS15 isoform 1 Chain: BP
Small ribosomal subunit protein uS9A Chain: BQ
Small ribosomal subunit protein eS17A Chain: BR
Small ribosomal subunit protein uS13A Chain: BS
Small ribosomal subunit protein eS19A Chain: BT
RPS20 isoform 1 Chain: BU
RPS25A isoform 1 Chain: BZ
RPS28A isoform 1 Chain: Bc
RPS29A isoform 1 Chain: Bd
Small ribosomal subunit protein RACK1 Chain: Bg
Ubiquitin-40S ribosomal protein S31 Chain: Bf
40S ribosomal protein S12 Chain: BM
Large ribosomal subunit protein uL2A Chain: AA
Large ribosomal subunit protein uL3 Chain: AB
RPL4A isoform 1 Chain: AC
RPL5 isoform 1 Chain: AD
Large ribosomal subunit protein eL6A Chain: AE
Large ribosomal subunit protein uL30A Chain: AF
Large ribosomal subunit protein eL8A Chain: AG
Large ribosomal subunit protein uL6A Chain: AH
RPL10 isoform 1 Chain: AI
RPL11A isoform 1 Chain: AJ
Large ribosomal subunit protein eL13A Chain: AL
Large ribosomal subunit protein eL14A Chain: AM
Large ribosomal subunit protein eL15A Chain: AN
Large ribosomal subunit protein uL13A Chain: AO
Large ribosomal subunit protein uL22A Chain: AP
Large ribosomal subunit protein eL18A Chain: AQ
Large ribosomal subunit protein eL19A Chain: AR
60S ribosomal protein L20 Chain: AS
Large ribosomal subunit protein eL21A Chain: AT
Large ribosomal subunit protein eL22A Chain: AU
Large ribosomal subunit protein uL14A Chain: AV
RPL24A isoform 1 Chain: AW
Large ribosomal subunit protein uL23 Chain: AX
Large ribosomal subunit protein uL24A Chain: AY
Large ribosomal subunit protein eL27A Chain: AZ
Large ribosomal subunit protein uL15 Chain: Aa
RPL29 isoform 1 Chain: Ab
Large ribosomal subunit protein eL30 Chain: Ac
Large ribosomal subunit protein eL31A Chain: Ad
RPL32 isoform 1 Chain: Ae
Large ribosomal subunit protein eL33A Chain: Af
Large ribosomal subunit protein eL34A Chain: Ag
Large ribosomal subunit protein uL29A Chain: Ah
Large ribosomal subunit protein eL36A Chain: Ai
Large ribosomal subunit protein eL37A Chain: Aj
RPL38 isoform 1 Chain: Ak
Large ribosomal subunit protein eL39 Chain: Al
Ubiquitin-ribosomal protein eL40A fusion protein Chain: Am
Small ribosomal subunit protein eS32A Chain: An
Large ribosomal subunit protein eL42A Chain: Ao
Large ribosomal subunit protein eL43A Chain: Ap
RPL1A isoform 1 Chain: E
18S Ribosomal RNA Chain: B5
25S ribosomal RNA Chain: A1
5s rRNA Chain: A3
5.8 S rRNA Chain: A4
Internal ribosome entry site Chain: EC

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.38Å
Relevant EMDB volumes: EMD-28632